Description

Cardiomyocyte Selective Medium (CSM) is a complete medium designed for the purification of cardiomyocyte cells derived from human pluripotent stem cells (hPSC). Current protocols allow for the differentiation of hPSC to cardiomyocytes at a high efficiency, however, the derived cells are a mixed population containing non-cardiomyocyte cells. The non-cardiomyocyte cells may interfere with downstream analyses. Density-gradient centrifugation, genetic modification, and cell sorting based on cell surface markers or mitochondria dyes have been applied to enrich for the hPSC-derived cardiomyocytes. These methods, however, either involve complicated procedures or require special equipment, and are not ideal for therapeutic purposes. Based on the unique metabolic properties of fetal and hPSC-derived cardiomyocytes, which can use lactate as a major energy source, CSM is developed to enrich for the hPSC-derived cardiomyocytes using an efficient, easy, and noninvasive approach. 

 

CSM is a serum-free medium specially designed for the selective growth of cardiomyocytes. It significantly improves the purity of hPSC-derived cardiomyocyte cell populations in 4 - 6 days, requiring only daily medium change. While culturing in the CSM, the non-cardiomyocyte cells were gradually eliminated, whereas the cardiomyocytes remain contracting in the culture. 

 

The CSM can also be used for cardiomyocytes derived from mouse pluripotent stem cell and/or the primary cells isolated from the hearts of rat neonatal pups.

 

Components

CSM consists of 500 mL of basal medium (Cat. #5911-b) and 10 mL of 50X Cardiomyocyte Selective Growth Supplement (CSGS, Cat. #5962).

 

Recommended Medium

 

Product Use

CSM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

 

Storage

Store the basal medium and complete CSM at 4°C, and the CSGS at -20°C. Protect from light.

 

Shipping

Dry ice.