上海中乔新舟生物科技有限公司是美国Sciencell公司官网公布的一级代理商: 美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。 ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。 请登录Sciencell公司官方网站(www.zqxzbio.com或www.sciencellonline.com)以确保购买正规公司产品。 产品:人关节软骨细胞 货号:4650  Description Chondrocytes are the resident cells of cartilage and are responsible for synthesizing a range of collagenous and non-collagenous extracellular matrix macromolecules. These include collagen type II, aggrecan and link protein and smaller amounts of collagen types IX and XI [1]. The control of proliferation and differentiation of chondrogenic cells is central to the coordinated development of the vertebrate skeleton. Chondrocytes are capable of producing and responding to a large number of peptide growth factors and cytokines, including insulin-like growth factor-1 and interleukin-1 [2]. Chondrocyte cultures are useful in vitro models for studying cartilage regeneration and repair, cytokine and growth factor effects on cartilage, specific gene regulation and pathophysiology of arthritis. HC-a from ScienCell Research Laboratories are isolated from human articular cartilage. HC-a are cryopreserved at primary cultures and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HC-a are characterized by the cytochemically detection of S100 and type II collagen. HC-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HC-a are guaranteed to further expand for 15 population doublings in the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Chondrocyte Medium (CM, Cat. No. 4651) for the culturing of HC-a in vitro. Product Use HC-a are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. Shipping Dry ice. Reference [1]. E. Kolettas, H. I. Muir, J. C. Barrett 1 and T. E. Hardingham (2001) Chondrocyte phenotype and cell survival are regulated by culture conditions and by specific cytokines through the expression of Sox-9 transcription factor. Rheumatology, 40(10): 1146-1156. [2]. Fosang A, Tyler JA, Hardingham TE, (1991) Effect of interleukin-1 and insulin-like growth factor-1 on the release of proteoglycan components and hyaluronan from pig articular cartilage in explant culture. Matrix 11:17-24. |