Preparation : | Produced in mice using highly pure (>98%) recombinant human TGFbeta1 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography. |
Catalog #: | 500-M66 |
Source : | Mice |
Formulation : | A sterile filtered antibody solution was lyophilized from PBS. |
Stability : | The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. Frozen aliquots are stable for at least six weeks at 2-8oC or for at least six months at -20oC. Avoid repeated freeze-thaw cycles. |
Reconstitution: | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. |
Applications : | |
Selected References : | Selected List of References Citing This PeproTech Product |
Neutralization | Data Not Available. |
Elisa | In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant human TGFβ1 when used in conjunction with compatible secondary reagents. |
Western Blot | To detect hTGFβ1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGFβ1 is 2.0-4.0 ng/lane, under non-reducing conditions.
In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with human TGFβ2 and TGFβ3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution. |
Country of Origin : | USA |
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| | Size B | 500μg | $150 |  | | |
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| Special Sizes:
Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request. |
| Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
