The GK1.5 monoclonal antibody specifically binds with the mouse CD4 molecule, also known as L3T4, a 55 kDa differentiation antigen which binds to the MHC class II. CD4 is expressed on most thymocytes, a subpopulation of mature T lymphocytes, dendritic cells, pluripotent hematopoietic stem cells, B cell precursors, and lymphoid precursors inside the thymus. It is also expressed on the mouse egg cell membrane, enhancing adhesion to MHC class II bearing sperm. By interaction with MHC class II on the surface of APC, CD4 initiates the development of T lymphocytes and helps the optimum functioning of mature T lymphocytes.The binding of the GK1.5 antibody blocks the binding of the Anti-Mouse CD4 RM4-5 antibody. 

Stability :

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20^oC. Frozen aliquots are stable for at least six weeks at 2-8^oC or for at least six months at -20^oC. Avoid repeated freeze-thaw cycles.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.

Applications :

Neutralization 

Data Not Available.

Elisa 

In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 800 pg/ml of recombinant human Eotaxin when used with PeproTech's biotinylated antigen affinity purified anti-human Eotaxin (500-P41GBt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.

Western Blot 

To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Country of Origin :

USA

Size B	500μg	$150

Special Sizes:
Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.

Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.