Monoclonal Antibodies
Anti-Murine EGF (Monoclonal Antibody)


 
		Preparation :
Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine EGF as the immunizing antigen.  This IgG2AK antibody was purified from cell culture by Protein G affinity chromatography.
		Catalog #:
500-M136
		Source :
Rats
		Formulation :
A sterile filtered antibody solution was lyophilized from PBS.
		Stability :
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. Frozen aliquots are stable for at least six weeks at 2-8oC or for at least six months at -20oC. Avoid repeated freeze-thaw cycles.
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.
		Applications :
 
		Neutralization 
To yield one-half maximal inhibition [ND50] of the biological activity of mEGF (0.1 ng/ml), a concentration of 1.0-1.5 μg/ml of this antibody is required.
		Elisa 
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant murine EGF when used with PeproTech's biotinylated antigen affinity purified anti-murine EGF (500-P174GBt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
		Western Blot 
To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 0.25-0.50 ng/lane under non-reducing conditions and 10.0-15.0 ng/lane under reducing conditions.
Country of Origin :
USA
        Size B
500μg
$150 
 
 
Special                     Sizes: 
 Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.
Usage:
For Research Use Only.  Not for use in diagnostic or therapeutic procedures.