Axon 800A 单细胞电穿孔仪 Molecular Devices
英文名称: Axon Axoporator 800A Electroporator
型号:: Axon Axoporator 800A
价格:请致电:010-57128832,18610462672
品牌: us    产品商标: axon/ molecular devices

Axon 800A 单细胞电穿孔仪
 
革命性单细胞电穿孔器 Axoporator™ 800A 电穿孔器,是世界上第一个商品化的单细胞电穿孔器,集中了生物物理学、细胞生物学以及分子生物学。这种以微量移液器为基础的输送系统使用电压脉冲暂时形成细胞膜孔,可以流过微量分子和/或离子。该装置与悬浮细胞大量电穿孔所使用的一样;通过电压脉冲使细胞膜的电介质破裂;但是单细胞的电穿孔与大量细胞同时电穿孔相比具有明显的优势:
  • 单个细胞可以进行具体的修改。无论您是否正在使用隔离的细胞,组织切片或者完整无缺的标本,这种技术使您可以只专注于单个细胞。
  • 细胞的精确区域可以有选择性的进行目标定位。顶部或者底部、神经突或者轴突、动物极与植物极-单细胞电穿孔使您在研究时只专注于细胞的具体区域。大型的、隔离的以及完整无缺的细胞器也适用于单细胞电穿孔。
  • 电穿孔只需要微小的体积。这有助于保护昂贵的和稀少的分子。.
  • 非常高的存活率,平均达80%。细胞可以较好的承受干预因为只有一小部分细胞膜与电压发送的微量移液器有关。因此细胞可以使用不同的基因进行不止一次的电穿孔。
成功案例记录
Axoporator 800A电穿孔器随着单细胞电穿孔前辈们的建议以及测试而不断发展。这些成功的协议都编成了文件放在用户手册以及Axoporator 800A的应用注释中。
Molecular Devices Axoporator 800A电穿孔器融合了Axon经过时间证明的两个强项:膜片钳技术和精确的波长生成。液晶显示屏、按钮和把手就组成了直观的用户界面。所有的脉冲和培训设置以及吸液管电阻和功率读数值在液晶显示器上都可以显示出来。如果需要的话,微量移液器的电压和电流也可以从BNC连接器上进行记录。提供了三种不同的脉冲类型,三种都可以以任何极性或者以任何顺序进行运行,这些包括:
  • 矩形 ''''方块''''
  • 双极
  • 双相 — 一种特殊的脉冲,经证明可以有效并且有效率的将基因按照14千字节的速度输送到细胞中。
灵活的控制和设计
尽管其设计是用于方便的单独使用,但是外部的波长生成器,例如pCLAMP软件以及Digidata 1322A可以作为脉冲指令源。
一旦配置好, Axoporator 800A可以通过按下按钮一次而激活。作为选择,也可以通过脚踏开关或者是外部的触发装置(TTL)进行激活,从而可以使用两手控制显微镜以及显微操纵器。 同步输出的BNC也可以用于使可选择的记录系统进行同步。
根据我们的经验,研究者永远可以找到非传统的、聪明的方法来使用我们的产品。因此Axon具有灵活设计的惯例。Axoporator 800A将两个非常重要的特征整合到将来预计的应用中:
  • 提供了一个“工厂型”输入,下载固件,按照发现进展的领域进行升级。.
  • 电压范围扩大到100伏,因为单细胞电穿孔最理想的电压在某些标本中可能是几个十几伏。
标准的AP-1A-1MU headstage可以通过最大达100微安的电流。可选择的AP-1A-0.1MU headstage可以通过最大达1豪安的电流。
更多便利
为了帮助定位微量移液器,内置音频***器在您接近细胞并接触时可以改变音调。这个音频***器和电阻显示一起,为在组织切片中盲目细胞搜索提供了一个有用的工具。
跟踪电穿孔数量的指令是快速的,因为有手动和自动两种计数器。
Axoporator的小脚使其非常容易定位在显微镜附近。
即装即用
不需要使用计算机。仅需显微镜、显微操纵器以及若干微量移液器即可构成完整的系统。
提供的附件
理论和操作指南
AP-1A-1MU headstage (或者在下定单的时候替换掉AP-1A-0.1MU)
SCE-1U细胞模型(可选择10兆欧/15兆欧)
一个HL-U电极夹持器
一个安装杆
脚踏开关
接地附件
订购信息
Axoporator 800A
  • 包括一台AP-1A-1MU 探头,可选择AP-1A-0.1MU 探头在订购时进行替换

f large directed libraries2 as well as novel assays including channel opener assays and multiplexing multiple ion channel targets in a single assay3. The IonWorks Barracuda System supports both PPC and single hole (SH) recording modes. SH recording mode is important where information must be obtained from individual cells during ion channel assay optimization and in applications such as the selection of individual clones for cell line optimization.

The IonWorks Barracuda System features a 384-well assay plate (our PatchPlate™ consumable) with a uniquely designed recording chamber that is optimized for rapid fluid exchange. This is especially important when measuring fast LGICs in order to resolve the rapid activation and desensitization kinetics of their ionic currents. The system combines 384 discreet amplifiers and a 384-channel pipettor head for the recording of ionic currents in all wells simultaneously. The parallel recording and fluidics configuration allow for the generation of over 10,000 data points per day. A typical ion channel assay with one compound addition per well can be completed in as little as 20 minutes. The system also allows up to eight compound additions per well further increasing the throughput and reducing the cost per data point. The IonWorks Barracuda System is ideal for primary screening of large directed compound libraries.  Further, with the capability to add multiple compound additions per well, for example increasing concentrations on a dose response curve, the system is also well suited for compound profiling and safety assessment.

The IonWorks Barracuda System comes in two configurations: a base system and a fully automated system which includes cell management capabilities and plate-handling. Both systems come with an automated pipettor for the transfer of cell suspension to a cell boat designed to minimize cell usage (<5mls/run) to cut down on the operating costs of cell culture. To load cells on the base system, the user simply provides a tube of cell suspension to a position on the assay deck. The automated system adds a plate handler that shuttles PatchPlates, pipette tips, and compound plates from the external landing pad to each deck position and removes them back to the landing pad as needed. Onboard cell storage and cell suspension preparation is provided on  the assay deck of the automated system. Cells are removed from the cell storage vessel, followed by solution exchange and cell concentration utilizing the on-board centrifuge. The fully automated configuration provides unattended operation for up to 6 hours.

 

Feature

·               Simultaneous compound addition and data acquisition allows the measurement of both voltage- and rapidly activating and desensitizing ligand-gated channels

·               Unique flow through recording chamber design enables rapid solution exchange

·               384 discrete amplifiers combined with a 384 channel pipettor head for parallel acquisition of an entire 384 well PatchPlate™ in as little as 20 minutes

·               Optional on-board cell management and plate handling for unattended operation up to six hours and >10,000 data points per day

·               CellPettor delivery of cell suspension minimizes on board cell usage when operating with or without optional cell management

·               Proven low-cost IonWorks PatchPlate consumable to keep running costs down

·               Eight compound additions per well further increases throughput and decreases the cost per data point over previous IonWorks systems

·               Population Patch Clamp™ (PPC) technology which reduces well-to-well variability to deliver consistent, reproducable results

·               Flexible software with two modes of operation, Assay Development and Screening streamlines assay optimization and simplifies ion channel screening

·               Rapid analysis of ionic currents using simple metrics similar to the IonWorks Quattro

·               Ultrasonic pipette tip washer with two wash solutions eliminates the potential of cross contamination across experimental runs

 Application

Voltage-gated ion channels:  Many voltage-gated ion channels (VGIC) including Na+, K+, Cl-, and Ca2+ channels have been measured on IonWorks systems. The same VGIC targets measured on the IonWorks® HT and Quattro systems can be measured on the IonWorks Barracuda system. 

Ligand-gated ion channels: The measurement of ligand-gated ion channels (LGIC) including ASIC1a, GABA, and nACh channels have been validated on the IonWorks Barracuda system.

Directed library screening: The introduction of the Population Patch Clamp (PPC) technique allowed the screening of large directed libraries in the 10s-100s of thousands of compounds against ion channel targets.

Clonal selection: The IonWorks family of instruments has been shown to be an excellent way of identifying and optimizing cell lines for use with IonWorks systems and other electrophysiological assays.    

Lead identification: Ion channel targets are implicated in many diseases. Once researchers determine that a particular ion channel is involved in a particular disease state, a screening assay can be established against which large numbers of compounds can be screened to identify "hits" or "leads" that could ultimately become drug candidates. 

Lead optimization: After "hits" or "leads" have been identified, drug discovery researchers optimize their chemical structure in order to get the best possible drug candidates-the compounds that are most likely to have the desired effect on ion channels with the fewest possible side effects.

Safety assessments: A critical part of lead optimization involves early safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns as early as possible. This allows researchers to focus their resources on the most promising drug candidates to streamline the drug discovery process.  Safety assessment assays including hERG, HCN, KCNQ, and Nav 1.5 channels have been published.