深层过滤纸板－瑞士Filtrox CH 20
英文名称： Depth Filter Sheet
型号:： CH 20（P）
价格:请致电：010-57128832,18610462672
品牌: 瑞士

深层过滤专家Filtrox源自于1938年瑞士的品质保证，PURAFIX深层过滤纸板主要应用于制药和其他高纯度要求，析出离子低，且释放致热源少，该系列所有纸板材料都通过FDA认证。

应用 Application
Fitrox纸板提供各种大小的孔径，因而其应用十分广泛。纸板孔径的级别从粗到细，从粗过滤到无菌过滤。
工业应用领域：
－API（活性药物成分）
－生物制药领域
－饮料和酒类生产
－酶制剂
－中草药或其他天然提取物
－制药中间体
－各类溶剂

纸板尺寸 Sheet sizes
PURAFIX＠纸板最大尺寸为1215×2425 mm，可以根据客户要求制成各种尺寸和形状，包括正方形，矩形，圆形，开孔/无孔，折叠等等。

纸板等级 Available grades
标准型               孔径 [um]      用途分类
CH 6 (P)            35－15          粗滤纸板
CH 9 (P)            30－10
CH 15 (P)          20－8.0
CH 20 (P)          15－6.0          澄清纸板
CH 30 (P)          12－5.0
CH 40 (P)          9.0－4.0
CH 50 (P)          6.0－3.0
CH 70 (P)          3.0－1.5         精滤纸板
CH 100 (P)        1.5－0.6         除菌纸板
CH ST 110 (P)   0.8－0.5         无菌纸板
CH ST 130 (P)   0.6－0.4
CH ST 140 (P)   0.4－0.2
CH ST 150 (P)   0.2－0.04

杀菌条件 Sterilization conditions
纸板可采用85℃热水或125℃蒸汽在线杀菌

材质 Material
漂白提纯纤维素，天然无机助滤剂，聚酰胺（<3％）。不含塑胶纤维、甲醛。纸板不含转基因物质，不含常见过敏原。

Filtrox质量保证体系 Filtrox quality assurance
采用国际标准的质量保证体系：
－ISO 9001（质量管理认证）
－ISO 14001（环境管理认证）
－HACCP（食品质量控制体系）
－FDA（美国食品药品管理局药监标号：16418）
Filtrox过滤纸板的外部测试符合：
－NAmSA的CFG标准

/table>用来产生这种疫苗的病毒并不长在人的肝脏中，现在RCCS培养的肝使产生肝炎疫苗的病毒生长在人的肝脏成为现实。RCCS在美国已广泛用于生产。 软骨再生：经培养的软骨密度极高，可治疗关节损伤。 ***、酶和其它由人体组织产生的蛋白以及基因工程：经培养的高分化的人体组织，其被刺激后能分泌治疗用的蛋白。例如经培养的神经组织产生的神经生长***能修复脊椎的损伤。 骨髓再生：培养的骨髓生长情况极佳，且可连续进行增生和低温冷冻保藏，可建大型骨髓库。 糖尿病：胰岛素培养后能插入体内继续生长，无数患者有望免去长期注射胰岛素。 有效杀灭肿瘤：对肿瘤组织活体取样，与自身的白细胞或淋巴细胞在RCCS中混合培养，刺激或驯化它们来识别和攻击肿瘤组织，然后把经驯化后有杀伤力的细胞直接注入病灶。这样，这些经感化的淋巴细胞彻底杀灭了肿瘤。 肿瘤、爱滋病、肾病和心脏病的理想模型：培养人的器官、腺体和淋巴结。然后感染这些类器官，再跟踪其生长。把药物用在被感染的经培养的组织模型上，研究其对抗疾病的效果及其对抗方式。例如肿瘤组织的培养有利于测试化疗药在病人自身的经培养和分化的肿瘤上的疗效，避免了用药的盲目性。以前这是在鼠的组织模型上进行的，但物种差异使许多肿瘤在鼠中的生长情况并不好。而在RCCS中，所有的肿瘤组织都完好生长，且避免了原先鼠蛋白的干扰。 皮肤移植：经培养的皮肤具有极高分化度避免了以前培养的皮肤在肌理、灵巧度和肤色的不足。 3.【我们所提供的各种培养系统的对比】　 　 剪切力 物质传递 维数 异类细胞的共同培养 细胞密度 (细胞/ml)
静态培养 (T 型烧瓶) 无 准二维 3至4层的二维 有限 0.3 - 1x106
静态基质培养 无 有限的三维 有限的三维 有限 低
转瓶培养 中 好的二维 3至4层的二维 极有限 1 - 5x106
搅拌悬浮培养 中/高 好 极有限的三维 极有限 106 - 107
空气升液培养 中/低 好 极有限的三维 极有限 106 - 108
空心纤维灌注培养 无 好 有限的三维 有限 107 - 108
旋转培养 (生物反应器) 极低 极佳 出色的三维 极佳 108
RCCS™ Consists of a single station Rotator Base, Power Supply, Operation Manual, and Reusable STLV or HARV Culture Vessel of your choice.* Also accepts Disposable Vessels.
RCCS™-4 Consists of a four station Rotator Base, Power Supply, Operation Manual, and four Reusable STLV or HARV Culture Vessel of your choice - you may select a variety of vessels with the system. All vessels do not have to be the same.* Accepts Reusable and Disposable Vessels.
STLV Vessels Reusable Vessel. Oxygenated by a center core silicone rubber gas transfer membrane. The STLV is intended for microcarrier cell culture and explant tissue cultures.* Available in 55, 110, 250, or 500 ml sizes
HARV Vessels Reusable Vessel. Oxygenation by a flat silicone rubber gas transfer membrane. Used to culture both suspension and anchorage dependent cells.* Available in 50 and 10ml sizes
How It Works Rotary Cell Culture System
Since The Rotary Cell Culture System has no impellers, airlifts, bubbles,or agitators, tissue damage from impact and turbulence is significantly decreased as compared to conventional bioreactors. Shear stress and damage is so low that it is essentially insignificant.

The operation of Synthecon''s RCCS differs from all other cell culture systems.The cylindrical culture vessel is filled with culture fluid and the cells or tissue particlesare added. All air bubbles are removed from the culture vessel. The vessel is attached to the rotator base and rotated about its horizontal axis. Tissues, cells or cell aggregates establish a fluid orbit within the culture medium in the horizontally rotating cylindrical vessel. The aggregates remain suspended in the culture medium having
little or no contact with the vessel wall.
A schematic view of the Perfused System, displaying medium vessel infusion mode. Perfuses media and nutrients in a single pass through the culture chamber from a media reservoir to waste. Also mode for refreshing media in the Recirculation mode.
As organoids grow in size, the rotational speed is adjusted to compensate for the increased settling rates of the larger particles. The motion of the vessel is sufficient to exchange nutrients, wastes and dissolved gases, and to allow cell-to-cell contacts that lead to aggregation.

Oxygen supply and carbon dioxide removal is achieved through a gas permeable silicone rubber membrane, which acts very much like the membranes of the lung. Since The Rotary Cell Culture System has no impellers, airlifts, bubbles, or agitators, tissue damage from impact and turbulence is significantly decreased as compared to conventional bioreactors. Shear stress and damage is so low that it is essentially insignificant. Under these conditions, cells communicate and synthesize extra cellular matrix as they would in vivo.

A schematic view of the Perfused System, displaying syringe-injection mode. In this mode flow is open to waste to compensate for displacement during injection. Allows for addition of reagents to supplement media.

Unlike cell and tissue cultures grown in two-dimensional flat plate systems, organoids grown in the Rotary Cell Culture System are functionally similar to tissues in the human body. You will be able to grow three-dimensional tissues in vitro that mimic the structure and function of the same tissue in vivo.

Tissue explants from biopsies or surgical procedures can be placed directly into the RCCS
maintaining their original cyto-architecture or can be expanded into tissue "organoids" or tumor models. This has important value in such fields as bone marrow, skin, pancreas and liver regeneration and the potential testing of chemotherapy drugs on the patient''s own tumor material ex vivo.

A schematic view of the Perfused System, displaying closed recirculation mode. Continuous flow of media and nutrients through a closed loop system, from the culture chamber advanced by the peristaltic pump, oxygenated via external silicone oxygenator and back into the culture chamber.

　
STLV - Exploded View Oxygenated by a center core membraneoxygenator, these reusable vessels areused for microcarrier cell cultureand explant tissue cultures.Shown here: exploded view　 STLV - Assembled Oxygenated by a center core membraneoxygenator, these reusable vessels areused for microcarrier cell cultureand explant tissue cultures.Shown here: assembled　
Advantages and Benefits of the Rotary Cell Culture System
　
The primary advantage of the RCCS over either dynamic or static tissue culture systems is that its gentle environment allows cells to aggregate, grow 3-dimensionally, and differentiate.
This advantage results in cells or tissues that very closely resemble the in vivo tissue quivalent.
In most dynamic systems, cells or tissues are suspend by physical forces that result in injury to the cells from contact with an impeller or by the shear forces caused by bubbles being sparged up through the culture for oxygenation and suspension.

These conditions cause injury to cells or tissues.

In static flat culture flasks or dishes the 2-dimensional environment and plastic substrate tend to alter gene expression and prevent differentiation.

Conversely, cells or tissues grown in the Rotary Cell Culture System br> are suspended by randomizing the gravity vector so they are, in effect, in continuous free-fall through the culture medium.

A good analogy would be to imagine a cell falling through a fluid-filled tube of infinite height. The tissue would fall, tumble, mix in the media and without any single gravity vector dictating growth direction; the tissue would grow in all directions. That is mechanism of the RCCS

Destructive stress forces are insignificant because this system has no propellers, airlifts, bubbles, or agitators. Because the cells are maintained in a gentle fluid orbit, the cells are allowed to co-localize, communicate with each other and form three-dimensional structures.

Furthermore, the RCCS is the only system currently available that allows researchers to introduce co-cultures that provide spontaneous tissue differentiation, higher cell multiplication, decreased cell death, and increased secretion of cell products.
Product Benefits
More than 50 cell and tissue types have been successfully cultured in the RCCS

Cells not only grow, but also develop into functional specialized tissue organoids. Scientists from academia, industry and government have published over 70 peer-reviewed articles utilizing RCCS technology the in scientific literature. These publications clearly show that the RCCS is the best technology available for growing differentiated mammalian tissue in vitro.
　
Benefits Include:
Wide Range of RCCS
Equipment Available, Within Any Laboratory Budget Three-Dimensional Tissues that Mimic Human Tissue in the Body Growth of Valuable Bio-Products from Tissue Reduced Costs of Culture Materials Simple Operating Procedures
Low Capital Investment Easy To Learn and Use Consistency of Results High-Density Cultures Rapid Culture Growth Harvesting Efficiency Wide Range of Uses Labor savings
　
Featured Tissues Grown Using the Rotary Cell Culture System
　
Brain
Neurons are being cultured in the RCCS to enable researchers to study various neurological diseases, e.g., Parkinson''s and Alzheimer''s.

　
Cancer Research
Research and phase 1 clinical trials are being conducted using the RCCS to investigate the growth and malignant changes of cancer as they develop from single cells to tumors including melanoma, prostate, breast and ovarian cancers, osteosarcoma, glioma, and colon cancers.

The RCCS model of three-dimensional cell culture enables examination of gene expression as a function of the stage of tumor cell aggregate growth. The Rotary Cell Culture System
is also being used in studies involving clinical cancer drug sensitivity testing,chemotherapy drug development, and cancer vaccine development.

　
Cartilage and Bone
Several institutions are investigating methods for regenerating and repairing bone and cartilage, including bone implants and grafts. These methods show promise for providing a therapy for skeletal disorders, and for allowing doctors to repair bone injuries or perform reconstructive surgery.
The RCCS creates a culture environment conducive to cell aggregation, and provides a powerful new tool for the study of bone physiology, biochemistry and gene expression.

　
Heart
Research also is being conducted to grow heart cells. In a specialized application televised recently on PBS, heart cells were shown growing in the RCCS bioreactor and actually beating under a microscope. This ability to grow heart tissue can greatly benefit open-heart surgeons who have to cut the pericardium. Often during healing, thepericardial sac actually adheres to the sternum.

Doctors who are using the RCCS are working towards creating a heart tissue "patch" to cover that wound thus eliminating adhesion to other organs.

　
Kidney
Kidney cells cultured in the RCCS bioreactor are being used for toxicity models and drug testing.

　
Liver
The RCCS provides a means to grow and expand hepatocyte cultures into high fidelity models of liver tissue. Liver tissue models will have a number of important applications including the design of gene therapy protocols, hepatocyte transplantation, the development of extra corporal assist devices, and test systems for the design of hepatitis vaccines and test of antiviral compounds.

In addition, researchers are conducting metabolism studies to determine how liver cells react to and metabolize toxins. Long-term hepatocyte cultures are also being maintained.

In conventional cultures, hepatocytes cannot be maintained for more than 10 to 15 days. Investigators using the RCCS however, have routinely maintained hepatocytes in vitro for as long as 70 days.

　
Pharmaceuticals
Valuable biopharmaceutical proteins for use in drugs are being produced in significantly higher quantities per cell using the RCCS

As a result, the need for larger, more costly bioreactors is reduced, as well as the volume of expensive materials that are required for protein production, and labor costs are significantly lowered.

　
Stomach
The RCCS bioreactor has helped create a gut model that''s being used in gastrointestinal absorption studies with biopharmaceuticals.
　
Tissue Explants
The U.S. Army Medical Research Institute of Infectious Diseases at Fort Detrick, Maryland, is using RCCS explants of human liver, spleen, and lymph nodes in an effort to find new compounds that could be used to treat patients infected with the lethal Ebola virus.

In other experiments, the RCCS has been able to preserve the normal three-dimensional structure of explanted human prostate tissue in culture. After an initial period of reorganization, explanted blocks of prostate tissue have been shown to maintain both tubuloglandular and fibromuscular stromal elements during six weeks of continuous RCCS culture. When added to the culture medium
of the bioreactor, malignant prostate epithelial cells can attach to and invade the normal fibromuscular stroma of the tissue. This provides a useful model for the study of host-tumor cell interactions in the human prostate.
　
The Future
SYNTHECON is growing smartly and strategically.
As the world''s leading manufacturer of 3-dimensional tissue culture systems, SYNTHECON is moving swiftly to expand awareness and demand throughout the scientific community of its leading-edge technology. And, it is committed to continually refining and improving its rotating cell culture systems for even broader applications.
Applications of the Rotary Cell Culture System
Scientists and clinicians worldwide are using the RCCS bioreactors for a wide variety of applications.

They are growing normal human tissue in vitro for testing of therapeutic drugs or for growing replacement tissue such as liver, skin, bone marrow, cartilage, heart muscle, and other tissues.

The RCCS bioreactor provides the research community with an excellent in vitro environment for culturing cells such as human tumor, viruses and cells that produce valuable bio-products including proteins, enzymes, hormones, antibodies and antigens.

Culture conditions in the RCCS also provide a superior in vitro environment for studying the effects of micro-environmental cues, especially intercellular communication on tissue-specific cell assembly, differentiation and function, and for studying phenotypic instability, drug/radiation-resistance, and the inter and intracellular signaling pathways involved in clonal expansion.

Synthecon''s RCCS bioreactors are being used at a number of prestigious research facilities.

The Cell & Gene Therapy Institute in San Antonio is using RCCS bioreactors to provide an inexhaustible supply of HIV infected human tissue for the development of HIV vaccines and test HIV pharmaceuticals.

The Food and Drug Administration uses the RCCS for intestinal parasite research.

The U.S. Army Medical Research Institute of Infectious Diseases at Ft. Detrick, Md.
has used the RCCS to grow Hong Kong Avian flu, Ebola, Monkey pox, Marbung and Hanta viruses in an effort to develop cures.