Pertussis toxin from Bordetella pertussis
英文名称: Pertussis toxin from Bordetella pertussis
型号:null    产品货号: P7208
价格:请致电:010-57128832,18610462672
品牌: sigma

 

产品名称

General description

Product is not activated. If activation is required, see Kaslow, et al., for suggested conditions.

Physical form

Lyophilized powder containing sodium chloride and sodium phosphate buffer salts

Application

The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.

Biochem/physiol Actions

Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.

性质

Related Categories BacteriaBacteria-derived adjuvantsBioactive Small MoleculesCell BiologyCell Culture,
form   lyophilized powder
storage temp.   2-8°C
Gene Information   Bordetella pertussis Tohama I ... ptxA(2665068), ptxB(2665069), ptxC(2665408)