Pertussis toxin from Bordetella pertussis 英文名称: Pertussis toxin from Bordetella pertussis | ||||||||
型号:null 产品货号: P7208 | ||||||||
价格:请致电:010-57128832,18610462672 | ||||||||
品牌: sigma | ||||||||
产品名称General descriptionProduct is not activated. If activation is required, see Kaslow, et al., for suggested conditions. Physical formLyophilized powder containing sodium chloride and sodium phosphate buffer salts ApplicationThe toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al. Biochem/physiol ActionsPertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels. 性质
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