美国ScienCell公司生产的内皮细胞培养基 Endothelial Cell Medium (ECM)是专门为正常人类微血管内皮细胞体外培养设计的最适于其生长的完全培养基。是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、***、生长因子、微量矿物质和低浓度胎牛血清(5%)。该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类微血管内皮细胞体外培养中的增殖和生长，并为其达到最理想营养平衡状态提供数量上和质量上的保证。

成分：
内皮细胞培养基包含 500 ml基础培养基；25ml胎牛血清(FBS，编号0025)；5ml内皮细胞生长添加物(ECGS，编号1052)和5 ml青霉素/链霉素溶液(P/S,编号0503) 。

Description

 

Endothelial cell medium (ECM) is a complete medium designed for optimal growth of normal human microvascular endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human microvascular endothelial cells in vitro.

Components

ECM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).

Product Use

ECM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Store the basal medium at 4°C, the ECGS, the FBS and the P/S solution at -20°C. Protect from light.