Disease: Endometrial Carcinoma G2 Origin: Derived from endometrial carcinoma Species: Homo sapiens Tissue: Uterus Morphology: Epithelial Properties: Adherent Cytogenic data: Tumorigenic Patient: Female, Asian Medium: IMDM + 10% FBS Subculture: 1:2 to 1:5 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility:Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD(typeB), NP, LDH DNA Profile (STR): D5S818:10,11,12; D13S317:9,10,11; D7S820:10,11,14; D16S539:11,12,13; VWA:17,20,22; TH01:7,9.3; Amelogenin:X; TPOX:11,14; CSF1PO:9,10 Mutations in HEC-59 Cells Related cells by Organ:HEC-59 cells HeLa cells SiHa cells CaSKi cells C33A cells References for HEC-59 cells:1. Hiramatsu, H. P., Kikuchi, Y., Seto, H., and Nagata, I. In vitro sensitivity of human endometrial cancer cell lines HEC-59 to paclitaxel or irinotecan (CPT-11) in combination with other aniticancer drugs. Anticancer Drugs, 11: 573-578, 2000. 2. Suzuki, T., Sasano, H., Sasaki, H., Fukaya, T., and Nagura, H. Quantitation of P450 aromatase immunoreactivity in human ovary HEC-59 during the menstrual cycle: relationship between the enzyme activity and immunointensity. J Histochem Cytochem, 42: 1565-1573, 1994. 3. Sasaki, H. [Aromatase activities of endometrial carcinomas HEC-59 and both basic and clinical analyses of endometrial hyperplasia as a premalignant disease]. Nihon Sanka Fujinka Gakkai Zasshi, 45: 763-775, 1993. 4. Tada, A., Sasaki, H., Nakamura, J., Yoshihama, M., and Terashima, Y. Aromatase activity and the effect of estradiol and *** on DNA synthesis in endometrial carcinoma cell lines HEC-59. J Steroid Biochem Mol Biol, 44: 661-666, 1993. 5. Yamamoto, T., Kitawaki, J., Urabe, M., Honjo, H., Tamura, T., Noguchi, T., Okada, H., Sasaki, H., Tada, A., Terashima, Y., and et al. Estrogen productivity of endometrium and endometrial cancer tissue; influence of aromatase on proliferation of endometrial cancer cells HEC-59. J Steroid Biochem Mol Biol, 44: 463-468, 1993. 6. Sasaki, H., Mitsunaga, S., Kitamura, T., Kishino, Y., Toyoizumi, T., Ogawa, M., Yamauchi, S., Iwata, M., Terashima, Y., and Mochizuki, S. [Radioresistant elements in adenocarcinoma of the uterus HEC-59]. Nihon Sanka Fujinka Gakkai Zasshi, 38: 1587-1594, 1986.