Origin: Embryonic kidney Species:Homo sapiens Tissue:Kidney Morphology:Epithelial Properties:Adherent Cytogenic data:transformed with adenovirus 5 DNA Medium:MEM + 2 mM Glutamine + 10% FBS Subculture: 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level:II Sterility:Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:HIV: Negative Hepatitis B: Negative Hepatitis C: NegativeReferences for HEK-293 cells:1. Graham FL, et al. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-72, 1977.