Disease: Breast adenocarcinoma Origin: Isolated from pleural effusions of a breast cancer patient. Cells exhibit some features of differentiated mammary epithelium including estradiol synthesis and formation of domes. Cells express both the wild type and variant estrogen receptors as well as progesterone receptor. Species:Homo sapiens Tissue:Breast, mammary gland Morphology:Epithelial Properties:Adherent Cytogenic data:2n = 46, hypertriploid to hypotetraploid. Patient:Female, Caucasian, 69 yrs of age Medium:EMEM (EBSS) + 2mM Glutamine + 0.01 mg/ml human recombinant insulin + 10% Foetal Bovine Serum (FBS). Subculture:1:2 to 1:6 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility:Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: estrogen receptor Isoenzymes: AK-1,1; ES-D, 1-2; G6PD, B; GLO-I, 1-2; PGM1, 1-2; PGM3,1 DNA Profile (STR): Amelogenin: X; CSF1PO:10; D13S317:11; D16S539:11,12; D5S818:11,12; D7S820:8,9; TH01:6; TPOX:9,12; vWA:14,15; Mutations in MCF-7 Cells Related cells by Organ:4T1 JIMT-1 MDA-MB-231 SK-BR-3 T47D MCF-7 References for MCF-7 cells:1. J Nat Cancer Inst 1973;51:1409.2. Cancer Res 1993;53:5882.