Disease: Glioblastoma (Multiform) Origin: Undifferentiated glioblastoma Species: Homo sapiens Tissue: CNS Properties: Adherent Cytogenic data: 5n+/-, Near-pentaploid 115+/- (104-126) Patient: Female, 67 yrs of age Medium: RPMI1640 + 10% FBS Subculture: 1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility:Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Mutations in SF-295 Cells References for SF-295 cells:1. Rutka JT, Giblin JR, Dougherty DY, Liu HC, McCulloch JR, Bell CW, Stern RS, Wilson CB, Rosenblum ML. Establishment and characterization of five cell lines derived from human malignant gliomas. Acta Neuropathol. 1987;75(1):92-103.