Disease: Basophilic leukemia  Origin: Chemically induced basophil Species: Rattus norvegicus , Rattus sp., Wistar Tissue: Peripheral blood Morphology: Fibroblast Properties: Adherent Medium: MEM + 15% FBS Subculture: 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility:Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:HIV: Negative Hepatitis B: Negative Hepatitis C: NegativeReferences for PBL-2H3 cells:1. Kulczycki A Jr., et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. 2. Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973.