英文名称： HYBOND-N+
| RPN119B | HYBOND-N+, 50X11.9X7.8CM |
| RPN119N | HYBOND-N, 50X11.9X7.8CM |
| RPN1210B | HYBOND-N+,20X12CMX10CM |
| RPN1210N | HYBOND-N, 20X12CMX10CM |
| RPN132B | HYBOND-N+, 50X132MM DISCS |
| RPN132N | HYBOND-N, 50X132MM DISCS |
| RPN132T | HYBOND-NX NYLON MEMBRANE 50X1 |
| RPN137B | HYBOND-N+, 50X137MM DISCS |
| RPN137N | HYBOND-N, 50X137MM DISCS |
| RPN137T | HYBOND-NX NYLON MEMBRANE 50X1 |
| RPN1510B | HYBOND-N+, 20X15CMX10CM |
| RPN1510N | HYBOND-N, 20X15CMX10CM |
| RPN1520B | HYBOND-N+, 10X15CMX20CM |
| RPN1520N | HYBOND-N, 20X15CMX20CM |
| RPN1520T | HYBOND-NX NYLON MEMBRANE 10 X |
| RPN1732B | HYBOND-N+, 50X132MM DISCS,GRID |
| RPN1737B | HYBOND-N+, 50X137MM DISCS,GRID |
| RPN1782B | HYBOND-N+, 50X82MM DISCS,GRIDD |
| RPN1787B | HYBOND-N+, 50X87MM DISCS,GRIDD |
| RPN2020B | HYBOND-N+, 10X20CMX20CM |
| RPN2020N | HYBOND-N, 10X20CMX20CM |
| RPN2020T | HYBOND-NX NYLON MEMBRANE 10 X |
| RPN203B | HYBOND-N+, 20CMX3M |
| RPN203N | HYBOND-N, 20CMX3M |
| RPN203T | HYBOND-NX NYLON MEMBRANE 20CM |
| RPN2222B | HYBOND-N+, 10X22CMX22CM |
| RPN2222N | HYBOND-N, 10X22CMX22CM |
| RPN2222T | HYBOND-NX NYLON MEMBRANE 10 X |
| RPN2250B | HYBOND N+ 50 X 22.2 X 22.2 CM |
| RPN303B | HYBOND-N+, 30CMX3M |
| RPN303N | HYBOND-N, 30CMX3M |
| RPN303T | HYBOND-NX NYLON MEMBRANE 30CM |
| RPN3050B | HYBOND-N+, 30CMX50CM |
| RPN82B | HYBOND-N+, 50X82MM DISCS |
| RPN82N | HYBOND-N, 50X82MM DISCS |
| RPN82T | HYBOND-NX NYLON MEMBRANE 50X8 |
| RPN87B | HYBOND-N+, 50X87MM DISCS |
| RPN87N | HYBOND-N, 50X87MM DISCS |
Amersham Hybond™-N+
- Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm2¾recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
- Recommended for all high density membrane arrays.
Amersham Hybond™-N+
Technical Information
Amersham Hybond™-N+ yields excellent sensitivity in conventional Southern blotting. Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation is best for maximum reproducibility. Hybond-N+ is not suitable for Alkali blotting and fixation.
- Multiple reprobing is possible without loss of membrane integrity.
- QC tested in genomic Southern blots using GE Healthcare products.
- Special sizes available on request.
) | ) | ) |
Human genomic DNA (1, 0.5, 0.125, 0.06 and 0.03 µg loadings) digested with HindIII and transferred to Hybond-N+. Hybridizaton with A. N-ras, B. p53, and C. bcl-2 labeled DNA probes and detected using Gene Images™ random-prime labeling with CDP-Star™ detection.
Membranes should be stored at room temperature in a clean dry atmosphere away from excessive heat, light, and noxious fumes. To prevent contamination, handle membranes using gloves or blunt-ended forceps. Hybond-N+ is stable for up to 3 years. Membranes should be kept in the bags in which they are received. Performance is consistent when stored under the recommended conditions.
| TECHNICAL SPECIFICATIONS | |
| Pore size | 0.45 µm |
| Nucleic acid size required | > 50 bp |
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=46939