英文名称： HiScreen Capto Q
| 28-9269-78 | HiScreen Capto Q |
| 28-9269-79 | HiScreen Capto S |
| 28-9269-80 | HiScreen Capto MMC |
| 28-9269-81 | HiScreen Capto Adhere |
| 28-9269-82 | HiScreen Capto DEAE |
HiScreen Capto IEX Columns
Technical Information
HiScreen Capto IEX columns are part of the process development platform available from GE Healthcare. Five prepacked HiScreen columns with Capto Q, Capto DEAE (strong and weak anion exchange, respectively), Capto S (strong cation exchange), Capto adhere (strong multimodal anion exchange) and Capto MMC (weak multimodal cation exchange) are available.
These columns are due to the small column volume, 4.7 ml and 10 cm bed height excellent tools for method optimization, parameter screening, robustness testing and convenient scaling up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale up.
If necessary, two columns can easily be connected in series to give a bed height of 20 cm.
Media prepacked in HiScreen Capto IEX columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.
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HiScreen Capto IEX columns, prepacked for convenient and robust process development.
| TECHNICAL SPECIFICATIONS | |
| Media | Capto Q Capto S Capto DEAE Capto MMC Capto adhere |
| Column volume | 4.7 ml |
| Column dimension | 0.77 × 10 cm |
| Recommended flow rates | Capto Q, Capto SP, Capto DEAE: 1.2-2.7 ml/min (150-350 cm/h) Capto adhere, Capto MMC: 1.2-2.3 ml/min (150-300 cm/h) |
| Maximum flow rates | Capto Q, Capto SP, Capto DEAE: 6 ml/min (700 cm/h) Capto adhere, Capto MMC: 4.7 ml/min (600 cm/h) |
| Column hardware pressure limit | 8 bar (0.8 MPa, 117 psi) |
| Maximum pressure over the packed bed during operation, Dp† | 3 bar (0.3 MPa, 44 psi) |
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Robustness study with overlay of absorbance curves at 280 nm for cycles 1, 5 and 10 using HiScreen Capto MMC. The differences in UV signal is due to the use of different sample preparations.
| Dynamic binding capacity (DBC) for BSA on HiScreen Capto MMC after 1, 5, and 10 cycles | |
| HiScreen Capto MMC | DBC (mg BSA/ml media) |
| New column | 40 |
| After cycle 5 | 40 |
| After cycle 10 | 41 |
| Difference
| 3% |