英文名称： HITRAP Q HP
| 17-1153-01 | HITRAP Q HP, 5 X 1 ML |
| 17-1154-01 | HITRAP Q HP 5X5 ML |
| 17-1351-01 | HITRAP PHENYL HP, 5 X 1ML |
| 17-1353-01 | HITRAP PHENYL FF (LS), 5X1ML |
| 17-1355-01 | HITRAP PHENYL FF (HS),5 X 1ML |
| 17-1357-01 | HITRAP BUTYL FF, 5 X 1 ML |
| 17-1359-01 | HITRAP OCTYL FF, 5 X 1 ML |
| 17-1408-01 | HITRAP DESALTING, 5X5ML |
| 17-5053-01 | HITRAP Q FF, 5 X 1 ML |
| 17-5054-01 | HITRAP SP FF, 5 X 1 ML |
| 17-5055-01 | HITRAP DEAE FF 5 X 1 ML |
| 17-5056-01 | HITRAP CM FF, 5 X 1 ML |
| 17-5079-01 | HITRAP RPROTEIN A FF, 5X1ML |
| 17-5079-02 | HITRAP RPROTEIN A FF, 2 X 1 ML |
| 17-5080-01 | HITRAP RPROTEIN A FF, 1X5ML |
| 17-5080-02 | HITRAP RPROTEIN A FF, 5 X 5 ML |
| 17-5110-01 | HITRAP IGM PURIFICATION 5X1ML |
| 17-5111-01 | HITRAP IGY PURIFICATION 1X5 ML |
| 17-5112-01 | HITRAP STREPTAVIDIN HP5X1 ML |
| 17-5130-01 | GSTRAP FF, 5X1ML |
| 17-5130-02 | GSTRAP FF, 2X1ML |
| 17-5130-05 | GSTRAP FF, 100 X 1 ML |
HiTrap™ IEX HP Columns
- Prepacked with Q Sepharose™ High Performance or SP Sepharose™ High Performance for high resolution purifications on 34-μm beads.
- Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
- Designed for use with a syringe, peristaltic pump and chromatography system such as ÄKTA™design.
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HiTrap™ IEX HP Columns
Technical Information
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HiTrap™ SP HP and HiTrap™ Q HP ion exchange columns are fast and convenient to use.
The ion exchange media packed in HiTrap™ ion exchange columns are based on Sepharose™ High Performance. The small particle size (34μm) allows fast adsorption and desorption even at high sample loadings and flow rates. SP Sepharose™ High Performance is a strong cation exchange medium and Q Sepharose™ High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.
| TECHNICAL SPECIFICATIONS | |
| HiTrap™ SP HP and HiTrap™ Q HP | |
| Media | SP Sepharose™ High Performance Q Sepharose™ High Performance |
| Column volume | 1 ml or 5 ml |
| Max. flow rate | |
| 1-ml column | 4 ml/min |
| 5-ml column | 20 ml/min |
| Recommended flow rate | |
| 1-ml column | 1 ml/min |
| 5-ml column | 5 ml/min |
| Max. back pressure | 3 bar (43 psi, 0.3 MPa) |
| Storage | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
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Separation of human serum proteins on a 1 ml HiTrap™ Q column using a one-step gradient. The separation conditions were optimised for the binding of serum components other than the IgG fraction, which passed through. The result of a subsequent SDS-electrophoresis analysis is shown below.
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SDS -PAGE electrophoresis with PhastSystem™ and PhastGel™ Gradient 8-25, Coomassie™ stained. The separation conditions were optimised for the binding of serum components other than the IgG fraction, which passed through. The result of a subsequent SDS-electrophoresis analysis is shown below.
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