TECHNICAL SPECIFICATIONS |
| nProtein A Sepharose 4 Fast Flow | Protein G Sepharose 4 Fast Flow |
Ligand | Native protein A | Recombinant protein G lacking albumin-binding region |
Ligand density | » 6 mg Protein A/ml | » 2 mg Protein G/ml |
Ligand coupling method | Cyanogen bromide activation | Cyanogen bromide activation |
Matrix | Highly cross-linked agarose, 4% | Highly cross-linked agarose, 4% |
Binding capacity | > 30 mg human IgG/ml medium | > 20 mg human IgG/ml medium |
pH stability | 3 to 9 (working) | 3 to 9 (working) |
Storage | 20% ethanol | 20% ethanol |
Storage temperature | 4°C to 8°C | 4°C to 8°C |