2-D Protein Extraction Buffer Trial Kit

Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. 2-D Protein Extraction Buffer offers a convenient way to prepare high-quality protein lysates. The buffers are designed to produce high spot resolution for 2-D gel analysis. Six buffers are available as a dry powder, which is rehydrated with the provided DILUENT. The optimal buffer will depend on the nature of your sample, and the 2-D Protein Extraction Buffer Trial Kit allows you to evaluate each extraction buffer to find the most suitable buffer for your sample

2-D Protein Extraction Buffer Trial Kit — Technical Specifications

Complete Packsize	50 ml
Application	This study presents a comparison between different protein extraction buffers to investigate the extraction capabilities and differences between the buffers Rat liver proteins were extracted using 2-D Protein Extraction Buffer-I to -VI, as well as a standard 2-D lysis reference buffer
Storage Conditions	2-D Protein Extraction Buffers at 15°C to 30°C DILUENTS at 2°C to 8°C
Wetted Part	No
Guidance	Total protein extraction from tissues and cell lines trial kit with six different buffers for optimization of the method for 10 ml solution of each buffer. The kit contains 2-D Protein Extraction 2-D Protein extraction buffer-I (Urea, NP-40) 2-D Protein extraction buffer-II (Urea, CHAPS) 2-D Protein extraction buffer-III (Urea, Thiourea, ASB-16, CHAPS) 2-D Protein extraction buffer-IV (Urea, Thiourea, SB 3-10, CHAPS) 2-D Protein extraction buffer-V (Urea, Thiourea, CHAPS) 2-D Protein extraction buffer-VI (Urea, Thiourea, NDSB 201, CHAPS) 1 x 7 ml DILUENT-I 1 x 14 ml DILUENT-II 2 x 12 ml DILUENT-III