RESOURCE ETH
英文名称: RESOURCE ETH
型号:: 17-1184-01
价格:请致电:010-57128832,18610462672
品牌: usa

17-1177-01 RESOURCE Q, 1 ML
17-1178-01 RESOURCE S, 1 ML
17-1179-01 RESOURCE Q, 6 ML
17-1180-01 RESOURCE S, 6 ML
17-1184-01 RESOURCE ETH 1 ML
17-1185-01 RESOURCE ISO 1 ML
17-1186-01 RESOURCE PHE 1 ML
17-1187-01 RESOURCE HIC TEST KIT
17-5166-01 MONO Q 5/50 GL
17-5167-01 MONO Q 10/100 GL
17-5168-01 MONO S 5/50 GL
17-5169-01 MONO S 10/100 GL
17-5170-01 MONO P 5/50 GL
17-5171-01 MONO P 5/200 GL
17-5177-01 MINI Q 4.6/50 PE
17-5178-01 MINI S 4.6/50 PE
17-5179-01 MONO Q 4.6/100 PE
17-5180-01 MONO S 4.6/100 PE
17-5181-01 SOURCE 15Q 4.6/100 PE
17-5182-01 SOURCE 15S 4.6/100 PE
17-5186-01 SOURCE 15PHE 4.6/100 PE

SOURCE™ Media/RESOURCE™ and Tricorn Columns for HIC

  • A range of high-resolution HIC media designed for low- to medium-pressure systems ranging from a peristaltic pump to ÄKTA™design systems and FPLC™ System.
  • Ideal for final polishing in a purification process.
  • High performance at low back pressure, even with high sample loads of up to 25 mg of protein.
  • Features ether (SOURCE™ 15ETH), isopropyl (SOURCE™ 15ISO) and phenyl (SOURCE™ 15PHE) hydrophobic ligands coupled to the 15 μm monodispersed rigid polystyrene/divinylbenzene matrix.
  • Prepacked 1-ml RESOURCE™ columns for fast and high-resolution separation are available individually in each of the three matrices or as a set in the RESOURCE™ HIC Test Kit.
  • SOURCE 15PHE 4.6/100 PE is a Tricorn™ column designed for high resolution at lab scale and for optimization studies when scaling up.

SOURCE™ Media/RESOURCE™ and Tricorn Columns for HIC

Technical Information


Click here to view this image in high resolution
SOURCE™ 15ETH, SOURCE 15ISO, SOURCE 15PHE in media packs and prepacked Tricorn™ and RESOURCE™ columns.

 

TECHNICAL SPECIFICATIONS
SOURCE™ Lab Packs
Binding capacity  >   25 mg/ml 
Matrix  Monodisperse polystyrene cross-linked with divinyl benzene 
Particle size  15 μm 
pH stability  2-12 (long term), 1-14 (short term) 
Chemical stability  Stable in common buffers, chaotropic
agents, detergents, and polar
organic solvents. 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
RESOURCE™ Prepacked columns
Bed volume  1 ml 
Bed dimensions  6.4 × 30 mm 
Max. pressure  15 bar (1.5 MPa, 217 psi) 
Max. flow rate*  9.6 ml/min 
SOURCE 4.6/100 PE columns (Tricorn™)
Bed volume  1.7 ml 
Bed dimensions  4.6 × 100 mm 
Max. pressure  40 bar (4 MPa, 580 psi) 
Max. flow rate*  5 ml/min 
* H2O at 25°C.
Column not suitable for use with ÄKTAprime™ plus system.

 

 

Click here to view this image in high resolution
The figure on the right shows four runs in an optimization series of eight runs to find the optimal running conditions for the purification of protein tyrosine phosphatase on SOURCE 15PHE 4.6/100 PE (Tricorn™). In the optimization series, the concentration of ammonium sulfate, gradient length, pH, flow rate and additives were varied. The activity was measured by p-nitrophenyl phosphate (pNPP) activity assay at 405 nm. In Run 8, the increase in concentration of ammonium sulfate from 1.5 M to 2.0 M and the increase of the length of the gradient resulted in a better separation.

tr>   Autoclavability    At 121°C, pH 7 for 30 minutes (gel only)    Antimicrobial agent    20% ethanol    Autoclavability    At 121°C, pH 7 for 30 minutes (gel only)    Antimicrobial agent    20% ethanol    Autoclavability    At 121°C, pH 7 for 30 minutes (gel only)    Antimicrobial agent    20% ethanol 

 

Click here to view this image in high resolution
Selectivity of the five different prepacked HiPrep Sephacryl™ HR columns.

 

TECHNICAL SPECIFICATIONS
Prepacked HiPrep™ Sephacryl HR Columns
Bed dimensions   
  HiPrep™ 16/60
  HiPrep™ 26/60 
16 × 600 mm
26 × 600 mm 
Bed volume   
  HiPrep™ 16/60
  HiPrep™ 26/60 
120 ml
320 ml 
Sample volume   
  HiPrep™ 16/60
  HiPrep™ 26/60 
< 5 ml
< 13 ml
 
Rec. linear flow rate  5-15 cm/h 
Max. linear flow rate  30 cm/h 
(H2O at 25°C)   
Max. pressure over the packed bed during operation  1.5 bar (22 psi, 0.15 MPa) 
Column hardware pressure limit  5 bar (73 psi, 0.5 MPa) 
Theoretical plates  5000 m-1 
Storage  20% ethanol 
Storage temperature  4°C to 30°C