17-5110-01	HITRAP IGM PURIFICATION 5X1ML
17-5111-01	HITRAP IGY PURIFICATION 1X5 ML
17-5112-01	HITRAP STREPTAVIDIN HP5X1 ML
17-5130-01	GSTRAP FF, 5X1ML
17-5130-02	GSTRAP FF, 2X1ML
17-5130-05	GSTRAP FF, 100 X 1 ML
17-5131-01	GSTRAP FF, 1X5ML
17-5131-02	GSTRAP FF 5 X 5 ML
17-5131-05	GSTRAP FF, 100 X 5 ML
17-5143-01	HITRAP BENZAMIDINE FF 5 X 1ML
17-5143-02	HITRAP BENZAMIDINE FF 2 X 1 ML
17-5144-01	HITRAP BENZAMIDINE FF 1 X 5 ML
17-5154-01	HITRAP DEAE FF 5 X 5 ML
17-5155-01	HITRAP CM FF, 5 X 5 ML
17-5156-01	HITRAP Q FF, 5 X 5 ML
17-5157-01	HITRAP SP FF, 5 X 5 ML
17-5158-01	HITRAP Q XL, 5 X 1 ML
17-5159-01	HITRAP Q XL, 5 X 5 ML
17-5160-01	HITRAP SP XL, 5 X 1 ML
17-5161-01	HITRAP SP XL, 5 X 5 ML
17-5162-01	HITRAP ANX FF (HIGHSUB)5 X 1ML
17-5163-01	HITRAP ANX FF(HIGHSUB) 5 X 5ML
17-5193-01	HITRAP PHENYL FF (HS), 5 X 5ML
17-5194-01	HITRAP PHENYL FF (LS), 5X 5ML
17-5195-01	HITRAP PHENYL HP, 5 X 5 ML
17-5196-01	HITRAP OCTYL FF, 5 X 5 ML
17-5197-01	HITRAP BUTYL FF, 5 X 5 ML
17-5247-01	HISTRAP HP 5 X 1 ML
17-5247-05	HISTRAP HP 100 X 1 ML
17-5248-01	HISTRAP HP 1 X 5 ML
17-5248-02	HISTRAP HP 5 X 5 ML
17-5248-05	HISTRAP HP 100 X 5 ML
17-5255-01	HISTRAP FF 5 X 5 ML
17-5255-02	HISTRAP FF 100 X 5 ML
17-5281-01	GSTRAP HP 5 X 1 ML
17-5281-05	GSTRAP HP 100 X 1 ML
17-5282-01	GSTRAP HP 1 X 5 ML
17-5282-02	GSTRAP HP 5 X 5 ML
17-5282-05	GSTRAP HP, 100 X 5 ML
17-5286-01	HISTRAP FF CRUDE 5X5 ML
17-5286-02	HISTRAP FF CRUDE 100X5 ML

HiTrap™ IgM Purification HP Column

• Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
• Purification of human IgM possible.
• Recovery of IgM is high with excellent activity retention.
• Complete instructions for use and method optimization are included, as are recommendations for sample preparation.
• A variety of connectors for syringe, peristaltic pump, and chromatography systems are included

HiTrap™ IgM Purification HP Column

Technical Information

HiTrap™ IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-mercaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

 

TECHNICAL SPECIFICATIONS
Ligand	2-mercaptopyridine
Ligand concentration	2 mg/ml medium
Matrix	Highly cross-linked agarose, 6%
Average particle size	34 μm
Binding capacity	5 mg human IgM/ml medium
Maximum flow rate	4 ml/min
Recommended flow rate	1 ml/min
pH stability	3-11 (long term), 2-13 (short term)
Storage	20% ethanol
Storage temperature	4°C to 8°C

 

Purification of a-Shigella IgM on HiTrap™ IgM Purification HP.

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