适用于96孔深孔板，“+”开口，1.2/2.2ml 方形孔，灭菌，化学耐受型
英文名称： Silicone mat

型号:： DPMS-9-IMP-WS+

价格:请致电：010-57128832,18610462672

品牌: 杭州

1 行业内最完整密封产品线，多种选择。2 产品易开合，可有效密封深孔板、样品管等，方便操作，节省时间。3 良好的密封性可防止多种溶剂的挥发。4 耐受宽泛的温度范围，适用于各种实验。5 抗化学腐蚀，并可用于低温保存。6 硅胶预开孔盖允许移液器吸头或探针进入孔内而不对盖子造成损坏。

nd-color: #ffff99; height: 15pt; border-top: windowtext; border-right: windowtext 0.5pt solid">17-5079-02 HITRAP RPROTEIN A FF, 2 X 1 ML 17-5080-01 HITRAP RPROTEIN A FF, 1X5ML 17-5080-02 HITRAP RPROTEIN A FF, 5 X 5 ML 17-5110-01 HITRAP IGM PURIFICATION 5X1ML 17-5111-01 HITRAP IGY PURIFICATION 1X5 ML 17-5112-01 HITRAP STREPTAVIDIN HP5X1 ML 17-5130-01 GSTRAP FF, 5X1ML 17-5130-02 GSTRAP FF, 2X1ML 17-5130-05 GSTRAP FF, 100 X 1 ML 17-5131-01 GSTRAP FF, 1X5ML 17-5131-02 GSTRAP FF 5 X 5 ML 17-5131-05 GSTRAP FF, 100 X 5 ML 17-5143-01 HITRAP BENZAMIDINE FF 5 X 1ML 17-5143-02 HITRAP BENZAMIDINE FF 2 X 1 ML 17-5144-01 HITRAP BENZAMIDINE FF 1 X 5 ML 17-5154-01 HITRAP DEAE FF 5 X 5 ML 17-5155-01 HITRAP CM FF, 5 X 5 ML 17-5156-01 HITRAP Q FF, 5 X 5 ML 17-5157-01 HITRAP SP FF, 5 X 5 ML 17-5158-01 HITRAP Q XL, 5 X 1 ML 17-5159-01 HITRAP Q XL, 5 X 5 ML 17-5160-01 HITRAP SP XL, 5 X 1 ML 17-5161-01 HITRAP SP XL, 5 X 5 ML 17-5162-01 HITRAP ANX FF (HIGHSUB)5 X 1ML 17-5163-01 HITRAP ANX FF(HIGHSUB) 5 X 5ML 17-5193-01 HITRAP PHENYL FF (HS), 5 X 5ML 17-5194-01 HITRAP PHENYL FF (LS), 5X 5ML 17-5195-01 HITRAP PHENYL HP, 5 X 5 ML 17-5196-01 HITRAP OCTYL FF, 5 X 5 ML 17-5197-01 HITRAP BUTYL FF, 5 X 5 ML 17-5247-01 HISTRAP HP 5 X 1 ML 17-5247-05 HISTRAP HP 100 X 1 ML 17-5248-01 HISTRAP HP 1 X 5 ML 17-5248-02 HISTRAP HP 5 X 5 ML 17-5248-05 HISTRAP HP 100 X 5 ML 17-5255-01 HISTRAP FF 5 X 5 ML 17-5255-02 HISTRAP FF 100 X 5 ML 17-5281-01 GSTRAP HP 5 X 1 ML 17-5281-05 GSTRAP HP 100 X 1 ML 17-5282-01 GSTRAP HP 1 X 5 ML 17-5282-02 GSTRAP HP 5 X 5 ML 17-5282-05 GSTRAP HP, 100 X 5 ML 17-5286-01 HISTRAP FF CRUDE 5X5 ML 17-5286-02 HISTRAP FF CRUDE 100X5 ML 17-5319-01 HISTRAP FF 5 X 1 ML 17-5319-02 HISTRAP FF 100 X 1 ML 17-5441-22 HITRAP CAPTO S, 5X1 ML 17-5441-23 HITRAP CAPTO S, 5X5 ML 17-5468-51 HiTrap Capto SP ImpRes 5x1mL 17-5468-55 HiTrap Capto SP ImpRes 5x5mL 17-5470-51 HiTrap Capto Q ImpRes 5x1mL 17-5470-55 HiTrap Capto Q ImpRes 5x5mL 17-6002-33 HITRAP IEX SELECTION KIT 18-1142-75 REC PROTEIN PURIF HANDBOOK 28-4010-81 HITRAP_HIPREP 1_16 CONNEC AKTA 28-4014-77 HISTRAP FF CRUDE KIT 28-4017-45 GSTRAP 4B, 5 X 1 ML 28-4017-46 GSTRAP 4B, 100 X 1 ML 28-4017-47 GSTRAP 4B, 1 X 5 ML 28-4017-48 GSTRAP 4B, 5 X 5 ML 28-4017-49 GSTRAP 4B, 100 X 5 ML 28-4058-44 HiTrap Capto Adhere 5x1ml 28-4058-46 5x5 ml Hitrap Capto adhere 28-4082-53 HITRAP MABSELECT, 5 X 1 ML 28-4082-55 HITRAP MABSELECT, 1 X 5 ML 28-4082-56 HITRAP MABSELECT, 5 X 5 ML 28-4082-58 HITRAP MABSELECT XTRA, 5X1ML 28-4082-60 HITRAP MABSELECT XTRA, 1X5ML 28-4082-61 HITRAP MABSELECT XTRA, 5X5ML

HiTrap™ rProtein A FF Columns

Quick, convenient preparative purification of monoclonal antibodies.
Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources.
Recombinant Protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification

HiTrap™ rProtein A FF Columns

Technical Information

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

HiTrap™ rProtein A 1 ml and 5 ml columns are packed with 1 ml and 5 ml of rProtein A Sepharose™ Fast Flow, respectively.

TECHNICAL SPECIFICATIONS

Column dimensions (i.d.x.h) 0.7 x 2.5 cm (1 ml)

1.6 x 2.5 cm (5 ml)

Ligand Recombinant protein A (E. coli)

Ligand coupling method Epoxy activation, thioether coupling

Ligand density » 6 mg rProtein A/ml medium

Matrix Highly cross-linked agarose, 4%

Average particle size 90 μm

Binding capacity » 50 mg human IgG/ml medium

Dynamic binding capacities † 23 mg mouse monoclonal IgG_2a per ml gel

12 mg mouse monoclonal IgG₁ per ml gel

11 mg monoclonal humanised IgG₄ per ml gel

Maximum back pressure 3 bar, 0.3 MPa, 44 psi

Maximum flow rate ‡ 4 ml/min (624 cm/h) for 1 ml column

20 ml/min (600 cm/h) for 5 ml column

Recommended working flow rate 1 ml/min (156 cm/h) for 1 ml column

5 ml/min (150 cm/h) for 5 ml column

Chemical stability All commonly used buffers

pH stability 3-10 (working), 2*-11 (short term)

Recommended flow rate 1 ml/ min (1-ml column)
5 ml/min (5-ml column)

Maximum flow rate 4 ml/min (1-ml column)
20 ml/min (5-ml column)

Temperature stability

Regular use +4-40°C

Storage +4-8°C

Storage buffer 20% ethanol

Storage 20% ethanol

Storage temperature 4°C to 8°C

* pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

† Capacity of HiTrap™ rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.

‡ Room temperature, aqueous buffers

Column: HiTrap™ rProtein A 1 ml. Flow rate: 1 ml/min.

Sample: cell culture supernatants.

SDS-PAGE on PhastSystem™ using PhastGel™ Gradient 10-15, silver stained

Lane 1: Low Molecular Weight Calibration Kit (LMW)
Lane 2: Starting material, diluted 10×
Lane 3: Flowthrough pool
Lane 4: Eluted IgG_2b pool

Purification of mouse IgG_2b from ascites on HiTrap™ rProtein A FF using a syringe.

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=12887

TECHNICAL SPECIFICATIONS
Column dimensions (i.d.x.h)	0.7 x 2.5 cm (1 ml)
	1.6 x 2.5 cm (5 ml)
Ligand	Recombinant protein A (E. coli)
Ligand coupling method	Epoxy activation, thioether coupling
Ligand density	» 6 mg rProtein A/ml medium
Matrix	Highly cross-linked agarose, 4%
Average particle size	90 μm
Binding capacity	» 50 mg human IgG/ml medium
Dynamic binding capacities †	23 mg mouse monoclonal IgG_2a per ml gel
	12 mg mouse monoclonal IgG₁ per ml gel
	11 mg monoclonal humanised IgG₄ per ml gel
Maximum back pressure	3 bar, 0.3 MPa, 44 psi
Maximum flow rate ‡	4 ml/min (624 cm/h) for 1 ml column
	20 ml/min (600 cm/h) for 5 ml column
Recommended working flow rate	1 ml/min (156 cm/h) for 1 ml column
	5 ml/min (150 cm/h) for 5 ml column
Chemical stability	All commonly used buffers
pH stability	3-10 (working), 2*-11 (short term)
Recommended flow rate	1 ml/ min (1-ml column) 5 ml/min (5-ml column)
Maximum flow rate	4 ml/min (1-ml column) 20 ml/min (5-ml column)
Temperature stability
Regular use	+4-40°C
Storage	+4-8°C
Storage buffer	20% ethanol
Storage	20% ethanol
Storage temperature	4°C to 8°C
* pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
† Capacity of HiTrap™ rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.
‡ Room temperature, aqueous buffers
Column: HiTrap™ rProtein A 1 ml. Flow rate: 1 ml/min.
Sample: cell culture supernatants.