未经处理、灭菌表面。经 -线灭菌。
尤其适于细菌检测。
不含内毒素、无DNase、DNA、RNase、无细胞毒性。 

Dextrin Sepharose High Performance/MBPTrap HP

• Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes
• MBPTrap HP 1 ml and 5 ml columns prepacked with Dextrin Sepharose™ High Performance give convenient, time-saving operation and reproducible results
• Physiological conditions and mild elution preserve the activity of the target protein
• Compatible with commonly used aqueous buffers and easily regenerated with 0.5 M NaOH
• Easy scale-up
• Convenient usage with a peristaltic pump, or chromatography system such as ÄKTA™ design

Dextrin Sepharose High Performance/MBPTrap HP

Technical Information

Dextrin Sepharose High Performance and MBPTrap HP 1 ml and 5 ml columns for affinity purification of recombinant proteins tagged with maltose binding protein

Dextrin Sepharose High Performance and prepacked MBPTrap HP columns provide convenient affinity purification of recombinant proteins tagged with maltose binding protein (MBP). Dextrin Sepharose High Performance has high binding capacity for MBP-tagged proteins. This robust and stable Sepharose High Performance based medium enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.

Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.

SDS-PAGE (reducing conditions) of the purification of MBP2*-paramyosin-d-Sal.

Automated purification of MBP2*-paramyosin-d-Sal using the two-step AC-GF protocol on MBPTrap HP 1 ml (AC) and HiLoad 16/60 Superdex 200 pg (GF).

详情请查询以下网址：http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=167322

TECHNICAL SPECIFICATIONS
Matrix	Rigid, highly cross-linked 6% agarose
Average particle size	34 μm
Ligand	Dextrin
Dynamic binding capacity1	Approx. 7 mg MBP- DSal/ml medium
	Approx. 16 mg MBP-bGal/ml medium
Chemical stability	Stable in all commonly used aqueous buffers, 0.5 M NaOH (regeneration and cleaning)
pH stability
working	> 7
short term	2¾13
Storage	4°C to 8°C in 20% ethanol
MBPTrap HP
Column volume	1 ml and 5 ml
Column dimensions	0.7 × 2.5 cm (1ml); 1.6 × 2.5 cm (5 ml)
Recommended flow rate2	1 ml/min (1 ml); 5 ml/min (5 ml)
Max. flow rate2	4 ml/min (1 ml); 20 ml/min (5 ml)
Max. pressure2	0.3 MPa, 3 bar
Storage	20% ethanol
Storage temperature	4°C to 8°C
1 Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-DSal (MBP2*-paramyosin-DSal, Mr approx. 70 000, multimer in solution), and MBP-bGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution) 2 H20 at room temperature