GSTPrep FF 16/10
英文名称: GSTPrep FF 16/10
型号:: 28-9365-50
价格:请致电:010-57128832,18610462672
品牌: 美国

28-9365-50 GSTPrep FF 16/10 1 ×20 ml

GSTPrep™ FF 16/10 Column - Easy Scale-up Purification

Technical Information


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GSTPrep™ FF 16/10 for fast and easy scale-up purification of GST-tagged recombinant proteins.

 

GSTPrep™ FF 16/10 are 20-ml HiPrep™ columns prepacked with Glutathione Sepharose™ 4 Fast Flow for scale-up purification of recombinant GST-tagged proteins, other glutathione S-transferases, and glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow in combination with HiPrep™ column hardware provides excellent flow properties.

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165074

 

TECHNICAL SPECIFICATIONS
Medium  Glutathione Sepharose™ 4 Fast Flow 
Column volume  20 ml 
Column dimensions  1.6 × 10 cm 
Recommended flow rate*  1-10 ml/min (30-300 cm/h) 
Max. flow rate*  10 ml/min (300 cm/h) 
Max. pressure over  the packed bed during operation*  1.5 bar (0.15 MPa, 22 psi) 
Column hardware pressure limit  5 bar (0.5 MPa, 73 psi) 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
* H2O at room temperature.
Note: Binding of GST to glutathione is flow-rate dependent and lower flow rates
often increase the binding capacity. This is important to consider, especially
during sample loading and elution.