PCR反应板
型号:： 
价格:请致电：010-57128832,18610462672
品牌: solarbio

关于PCR(聚合酶链式反应）是利用DNA在体外摄氏95°高温时变性会变成单链，低温（经常是60°C左右）时引物与单链按碱基互补配对的原则结合，再调温度至DNA聚合酶最适反应温度（72摄氏度左右），DNA聚合酶沿着***酸到五碳糖(5'-3')的方向合成互补链。基于这个原理PCR仪实际就是一个温控设备，能够瞬间在变性温度，复性温度，延伸温度之间很好地进行控制，这时候PCR管或者PCR板是这个实验过程中必不可少的耗材。实验中对于PCR管的要求是管壁需要PCR仪的温度能够迅速均匀的传达到PCR管中的液体里面，所以PCR管管壁需要尽量薄，而且质地均匀，索莱宝公司提供多种规格的PCR管，如0.1ml，0.2ml，棕色等，满足老师不同的实验需求。PCR板通常有带不带裙边的不能规格，这个裙边部分跟PCR实验本身没什么关系，有没有裙边主要是根据实验中使用的仪器的型号决定的，带有一定厂家垄断PCR耗材的嫌疑，比如有些测序仪上只能匹配半裙边的96孔板，由于市场提供较少，需要使用厂家配套的PCR耗材，提高了实验成本。而一般的PCR仪使用带不带裙边的板子都是一样的，这样大家就能找到更物美价廉的PCR耗材。服务热线：陈天添 手机/微信13439225985 QQ609810384实验中涉及到的PCR其它相关耗材：YA0600 96孔PCR反应板 国产 Solarbio 20个 包YA0700 PCR八联排管 国产 Solarbio 200个 包YA0701 0.5mlPCR管 国产 Solarbio 1000个 包YA0702 0.2mlPCR管（平盖）国产 Solarbio 1000个 包YA0703 0.2mlPCR管（鼓盖）国产 Solarbio 1000个 包YA0800 96孔PCR管盒（适用于0.2mlPCR管）黄色 国产  Solarbio 1个 包PCR-0.2-C  PCR管(离心管） Axygen 0.2mlPCR-0.2D-C  PCR管(离心管）鼓盖  Axygen 0.2mlPCR-0.5-C  PCR管(离心管） Axygen 0.5mlPCR-0208-C  PCR八连排管(平) Axygen 0.2mlPCR-0208-P-C  PCR八连排管(鼓) Axygen 0.2mlPCR-TS  进口封板膜 Axygen 100张/盒AM-96-PCR-RD  96孔PCR硅胶封板膜 Axygen 10个/包PCR-96M2-HS-C  96孔PCR板 半裙边 Axygen 96孔 块服务热线：陈天添 手机/微信13439225985 QQ609810384

; border-top: windowtext; border-right: windowtext 0.5pt solid">HiScale 50 40BH 28-9644-45 HiScale 50 20BH

HiScale™ Empty Columns

Technical Information


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HiScale 16/40, 26/40, and 50/40 columns are supplied, complete with two adapters and Valco unions for easy connection to chromatography systems

 

HiScale™ columns are designed to withstand pressures of up to 20 bar (290 psi) and high flow rates, making them compatible with modern BioProcess chromatography media such as MabSelect™ and Capto™. The user-friendly design allows for an optimized process, through simplified and reproducible operation.

 

The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments as well as disassembly and cleaning. The measurement scale on the column makes it easier to predict and reach a predefined packing height, thereby increasing process control as well as reproducibility. Although optimized for high-pressure media, HiScale columns are fully compatible with all applications (and packing methods) currently applicable to XK columns.

 

HiScale columns are delivered with a column tube, outer protection tube, two adapters, PEEK tubing, and unions at both ends for easy connection to valves, pumps, and monitors.

 

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Purification and scale-up of IgG on MabSelect SuRe™ affinity chromatography medium (above) and scale-up of GFP on Capto Q IEX chromatography medium (below). The chromatogram on the top is a normalized overlay of UV absorbance (280 nm) for HiScreen™, packed with MabSelect SuRe (blue) and HiScale 16/40 packed with the same medium (red). The second chromatogram is a normalized overlay of UV absorbance (490 nm) for HiScreen Capto Q (blue) and HiScale 16/40 packed with Capto Q (red). The figure shows the excellent reproducibility during scale-up from HiScreen to HiScale with two different chromatographic techniques.

 

TECHNICAL SPECIFICATIONS
Column dimensions (i.d. × H) 
HiScale 16/20  16 mm × 20 cm  
HiScale 16/40  16 mm × 40 cm 
HiScale 26/20  26 mm × 20 cm 
HiScale 26/40  26 mm × 40 cm 
HiScale 50/20  50 mm × 20 cm 
HiScale 50/40  50 mm × 40 cm 
Max operating pressure   20 bar (290 psi) 
Operating temperature   4°C to 40°C 
pH stability  1-14 
Materials in contact with eluent  Polypropylene, borosilicate glass, polyamide, fluoro-rubber, PEEK capillary tubing, and Tefzel™ ferrules. 
Mesh size of net  10 μm, 20 μm, and 80 μm* nylon 

 

*HiScale 50 columns only

 

TECHNICAL SPECIFICATIONS - Maximum Bed Volumes and Bed Height Ranges
Column  Max. bed volume (ml)  Bed height range (cm) 
HiScale 16/20  40  0-20 
HiScale 16/40  80  8-40 
HiScale 26/20  106  0-20 
HiScale 26/40  212  13-40 
HiScale 50/20  393  0-20 
HiScale 50/40  785  14-40 

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