28-9365-41	HiPrep DEAE FF 16/10
28-9365-42	HiPrep CM FF 16/10
28-9365-43	HiPrep Q FF 16/10
28-9365-44	HiPrep SP FF 16/10
28-9365-45	HiPrep Phenyl FF (high sub) 16
28-9365-46	HiPrep Phenyl FF (low sub) 16/
28-9365-47	HiPrep Butyl FF 16/10
28-9365-48	HiPrep Octyl FF 16/10
28-9365-49	HiPrep Heparin FF 16/10
28-9365-50	GSTPrep FF 16/10
28-9365-51	HisPrep FF 16/10
28-9365-52	HiPrep IMAC FF 16/10

HiPrep™ IEX FF 16/10 Columns

• Q, SP, DEAE, and CM Sepharose™ 6 Fast Flow in prepacked 20-ml HiPrep™ columns provide fast, reliable, and reproducible preparative ion exchange separations.
• Ideal for rapid enrichments during initial capture of proteins from starting material.
• Simple operation.
• Compatible with single-pump configurations and other chromatography systems such as ÄKTA™design

HiPrep™ IEX FF 16/10 Columns

Technical Information

HiPrep™ Q FF16/10 , HiPrep™ SP FF 16/10, HiPrep™ DEAE FF 16/10 and HiPrep™ CM FF 16/10 combine good flow rates and high binding capacity.

 

TECHNICAL SPECIFICATIONS
Media	Q Sepharose™ Fast Flow SP Sepharose™ Fast Flow DEAE Sepharose™ Fast Flow CM Sepharose™ Fast Flow
Column volume	20 ml
Column dimensions	1.6 × 10 cm
Recommended flow rate*	2-10 ml/min (60-300 cm/h)
Max. flow rate*	10 ml/min (300 cm/h)
Max. pressure over the packed bed during operation*	1.5 bar (22 psi, 0.15 MPa)
Column hardware pressure limit	5 bar (73 psi, 0.5 MPa)
Storage	20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP)
Storage temperature	4°C to 30°C
*H2O at room temperature.

 

Separations of standard proteins on HiPrep™ CM FF 16/10 at pH 5.0, pH 6.5, and
pH 7.5.

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