英文名称： SUPERDEX PEPTIDE PC 3.2/30
| 17-1455-01 | PRECISION COLUMN HOLDER |
| 17-1458-01 | SUPERDEX PEPTIDE PC 3.2/30 |
| 17-1550-01 | COLUMN HOLDER, 10 CM |
| 18-1015-28 | PROTECTIVE CAPS (2/PK) |
| 17-5057-01 | MICRORPC C2-C18 ST 4,6-100 |
| 17-5068-01 | SOURCE 15RPC ST 4.6/100 |
| 17-5116-01 | SOURCE 5RPC ST 4,6-150 |
| 17-5172-01 | SUPEROSE 6 10/300 GL |
| 17-5173-01 | SUPEROSE 12 10/300 GL |
| 17-5174-01 | SUPERDEX 75 10/300 GL |
| 17-5175-01 | SUPERDEX 200 10/300 GL |
| 17-5176-01 | SUPERDEX PEPTIDE 10/300 GL |
| 28-9065-61 | Superdex 200 5_150 GL |
| 28-9205-04 | Superdex 75 5_150 GL |
| 17-0506-01 | PP COLUMN MONO Q HR 16/10 |
| 17-0507-01 | PP COLUMN MONO S HR 16/10 |
Superdex™ Peptide Columns
- High-resolution separation of peptides and other small biomolecules of 100-7000 molecular weight. The only product available in the industry that offers this fractionation range.
- Steep selectivity curve, high pore volume, and novel cross-linked agarose/*** matrix give exceptional performance.
- Powerful alternative and complement to traditional reversed phase chromatography of small peptides.
- Tricorn™ high-performance column (10/300 GL) expertly packed with Superdex™.
- Precision columns (PC) for micropurification and analysis¾10-fold smaller column volume than Tricorn™ columns.
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Superdex Peptide, 75 and 200 prepacked in Tricorn columns offer unmatched high-performance gel filtration.
| TECHNICAL SPECIFICATIONS | |
| Superdex™ Peptide | |
| Fractionation range (Mr) | 100-7000 |
| Matrix | Spherical composite of cross-linked agarose and *** |
| Average particle size | 13 μm |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning-in-place) |
| pH stability | 1-14 (working, long and short term) |
| Prepacked Superdex™ Peptide Columns* | |
| Bed volume | |
| PC 3.2/30 | 2.4 ml |
| Tricorn 10/300 GL | 24 ml |
| Sample volume | |
| PC 3.2/30 | 2-25 μl |
| Tricorn 10/300 GL | 25-250 μl |
| Max. pressure | |
| PC 3.2/30 | 20 bar (290 psi, 2 MPa) |
| Tricorn 10/300 GL | 18 bar (261 psi, 1.8 MPa) |
| Max. flow rate (H2O at 25°C) | |
| PC 3.2/30 | 150 μl/min |
| Tricorn 10/300 GL | 1.2 ml/min |
| Theoretical plates | > 30 000 m-1 |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Columns are not suitable for use with ÄKTAprime™ plus system.
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Separation of standard peptides on Superdex™ Peptide 10/300 GL.
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The separation of SPI peptides C0070 illustrates the difference in resolution for small peptides.The relative pore volume of Superdex™ Peptide is 55.5% compared to 39.1% for TSK G2000 SW.
Superdex™ Peptide Columns
Technical Information
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Reference
- Fägerstam, L. et al. Detection and characterisation of oligosaccharides in column effluents using surface plasmon resonance. Fourteenth International Symposium on the Separation and Analysis of Proteins, Peptides, and Polynucleotides, Heidelberg, Germany (1994).
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