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Separation of standard proteins on Tricorn™ Superdex™ 200.
| COLUMN HR 英文名称： COLUMN HR | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 型号:： 18-1000-98 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 价格:请致电：010-57128832,18610462672 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 品牌: 美国 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
HR (High Resolution) Columns
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=13485 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn™ Column Family, that provide excellent reproducibility and durability in purification using a chromatography system.
| TECHNICAL SPECIFICATIONS | |
| Superdex™ | |
| Exclusion limit (Mr) | |
| Superdex™ 75 | 1 × 105 globular protein |
| Superdex™ 200 | 1.3 × 106 globular protein |
| Separation range (Mr) | |
| Superdex™ 75 | 3000-70 000 globular protein |
| Superdex™ 200 | 10 000-600 000 globular protein |
| Matrix | Spherical composite of cross-linked agarose and *** |
| Average particle size | 13 μm |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place) |
| pH stability | 3-12 (working and long term), 1-14 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Superdex™ 10/300 GL Columns (Tricorn)* | |
| Bed dimensions | 10 × 300 mm |
| Recommended sample volume | 25-250 μl |
| Bed volume | 24 ml |
| Max. pressure | |
| Superdex™ 75 | 18 bar (261 psi, 1.8 MPa) |
| Superdex™ 200 | 15 bar (217 psi, 1.5 MPa) |
| Max. flow rate (H2O at 25°C) | |
| Superdex™ 75 | 1.5 ml/min |
| Superdex™ 200 | 1.0 ml/min |
| Theoretical plates | > 30 000 m-1 |
| Superdex™ PC 3.2/30 Columns | |
| Bed dimensions | 3.2 × 300 mm |
| Bed volume | 2.4 ml |
| Recommended sample volume | 2-25 μl |
| Max. pressure | |
| Superdex™ 75 | 24 bar (348 psi, 2.4 MPa) |
| Superdex™ 200 | 12 bar (174 psi, 1.2 MPa) |
| Max. flow rate (H2O at 25°C) | |
| Superdex™ 75 | 0.100 ml/min |
| Superdex™ 200 | 0.100 ml/min |
| Theoretical Plates | > 30 000 m-1 |
| Superdex™ 5/150 column (Tricorn™) | |
| Bed volume | 3 ml |
| Bed dimensions | 5 × 150 mm |
| Recommended sample volume | 4 - 50 μl |
| Recommended flow rate | 0.15 - 0.6 ml/min |
| Max. pressure | |
| Superdex 75 | 1.8 MPa |
| Superdex 200 | 1.5 MPa |
| Max. flow rate (H20 at 25°C) | |
| Superdex 75 | 0.7 ml/min |
| Superdex 200 | 0.8 ml/min |
| Theoretical plates | > 25000 m-1 |
| * Columns are not suitable for use with ÄKTAprime™ plus system.
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Separation of standard proteins on Tricorn™ Superdex™ 200.
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Separation of monomer and dimer on Tricorn™ Superdex™ 75.
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Column performance test with a set of standard proteins on Superdex™ 75
5/150 GL.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165422