COLUMN HR
英文名称： COLUMN HR
型号:： 18-1000-98
价格:请致电：010-57128832,18610462672
品牌: 美国

18-1000-98 COLUMN HR 16/5
18-1031-47 BOTTOM PIECE COMPLETE, XK 16
18-1031-48 BOTTOM PIECE COMPLETE, XK 26
18-1031-49 BOTTOM PIECE COMPLETE, XK 50
18-1031-80 PLUNGER, XK 16
18-1031-81 PLUNGER, XK 26
18-1101-28 FILTER 26 MM KIT
18-1152-82 TRICORN O-RING PACKING KIT
18-1153-01 TRICORN 5 COLUMN BOTTOM UNIT
18-1153-02 TRICORN 5 FILTER KIT
18-1153-03 TRICORN O-RING 3X1 PFR
18-1153-04 TRICORN 5/20 GLASS TUBE
18-1153-05 TRICORN 5/50 GLASS TUBE
18-1153-06 TRICORN 5/100 GLASS TUBE
18-1153-07 TRICORN 5/150 GLASS TUBE
18-1153-08 TRICORN 5/200 GLASS TUBE
18-1153-10 TRICORN 10 COLUMN BOTTOM UNIT
18-1153-11 TRICORN 10 FILTER KIT

HR (High Resolution) Columns

  • Compatible with any high-performance system including ÄKTA™design systems and FPLC™ System, HR columns provide precision sample application and elution, and excellent physical and chemical stability.
  • HR 16 columns are available in three different lengths: 5, 10, and 50 cm.
  • No metal parts¾glass construction allows visual observation at all times.
  • Wide range of accessories, adapters, and replacement parts available.

  • HR (High Resolution) Columns

    Technical Information


    TECHNICAL SPECIFICATIONS
    Max. pressure    
    HR 16  30 bar (3 MPa, 430 psi) 
    pH stability  1-14 
    Chemical stability  Resistant to most solutions  used in liquid chromatography  except hydrocarbons, aromatic  solvents, and chlorinated  hydydrocarbons. 

     

    TECHNICAL SPECIFICATIONS - HR Columns Bed Volumes and Heights
      Column Size  With One Adapter  With Two Adapters 
      i.d. Recommended Volume Bed Height Volume Bed Height
      (mm)   Bed Volume (ml)  (ml)  (cm)  (ml)  (cm) 
    HR 16/5  16  10  5.43-13.67  2.7-6.8  0.00-13.87  0.0-6.9 
    HR 16/10  16  20  14.3-22.3  7.1-11.1  6.2-22.3  3.1-11.1 
    HR 16/50  16  100  95.0-103.0  47.5-51.5  87.0-103.0  43.5-51.5 

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=13485

PE 17-5180-01 MONO S 4.6/100 PE 17-5181-01 SOURCE 15Q 4.6/100 PE 17-5182-01 SOURCE 15S 4.6/100 PE 17-5186-01 SOURCE 15PHE 4.6/100 PE 18-4271-00 O RING 64.5X3 EPDM GLASS TUBE 18-4284-01 FILTER TOOL, 60 MM 18-4610-60 DISTRIBUTION UNIT 60 MM TI 1 18-4610-62 O RING 55X1.6 EPDM

Superdex™ 75 / Superdex™ 200 Columns

Superdex™ 75 / Superdex™ 200 Columns

Technical Information


Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.

 

Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.

 

Superdex columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn™ Column Family, that provide excellent reproducibility and durability in purification using a chromatography system.

 

TECHNICAL SPECIFICATIONS
Superdex™
Exclusion limit (Mr) 
Superdex™ 75  1 × 105 globular protein 
Superdex™ 200  1.3 × 106 globular protein 
Separation range (Mr) 
Superdex™ 75  3000-70 000 globular protein 
Superdex™ 200  10 000-600 000 globular protein 
Matrix   Spherical composite of cross-linked agarose and *** 
Average particle size  13 μm 
Chemical stability  Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning in place) 
pH stability  3-12 (working and long term), 1-14 (short term) 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
Superdex™ 10/300 GL Columns (Tricorn)*
Bed dimensions  10 × 300 mm 
Recommended sample volume  25-250 μl 
Bed volume  24 ml 
Max. pressure   
Superdex™ 75  18 bar (261 psi, 1.8 MPa) 
Superdex™ 200  15 bar (217 psi, 1.5 MPa) 
Max. flow rate (H2O at 25°C) 
Superdex™ 75  1.5 ml/min 
Superdex™ 200  1.0 ml/min 
Theoretical plates  > 30 000 m-1 
Superdex™ PC 3.2/30 Columns
Bed dimensions  3.2 × 300 mm 
Bed volume  2.4 ml 
Recommended sample volume  2-25 μl 
Max. pressure   
Superdex™ 75  24 bar (348 psi, 2.4 MPa) 
Superdex™ 200  12 bar (174 psi, 1.2 MPa) 
Max. flow rate (H2O at 25°C) 
Superdex™ 75  0.100 ml/min 
Superdex™ 200  0.100 ml/min 
Theoretical Plates  > 30 000 m-1 
Superdex™ 5/150 column (Tricorn™)
Bed volume  3 ml 
Bed dimensions  5 × 150 mm 
Recommended sample volume  4 - 50 μl 
Recommended flow rate  0.15 - 0.6 ml/min  
Max. pressure   
Superdex 75  1.8 MPa 
Superdex 200  1.5 MPa 
Max. flow rate (H20 at 25°C)   
Superdex 75  0.7 ml/min 
Superdex 200  0.8 ml/min 
Theoretical plates  > 25000 m-1 
* Columns are not suitable for use with ÄKTAprime™ plus system.

 

 

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Separation of standard proteins on Tricorn™ Superdex™ 200.

 

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Separation of monomer and dimer on Tricorn™ Superdex™ 75.

 

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Column performance test with a set of standard proteins on Superdex™ 75
5/150 GL.

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165422