植物血球凝集素 P(PHA-P)
英文名称: Lectin from Phaseolus vulgaris (red kidney bean)
型号:null    产品货号: L8754
价格:请致电:010-57128832,18610462672
品牌: 美国
试剂级别: 生化级

Descriptions

Analysis Note  
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
 
Biochem/physiol Actions  
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.
 
Properties
form 
lyophilized powder
 
potency 
<62.5 μg per mL agglutination activity
 
 
<10 μg/mL mitogenic activity
 
total impurities 
salt, free
 
storage temp. 
2-8°C