biolog 1005 YT鉴定板 用于酵母菌的鉴定 美国BIOLOG公司独家研发的产品 ECO板,特别为微生物群落分析和生态研究设计,Eco板上包含国际土壤微生物学家精选的用于微生物群落分析最常用的31种碳源,是国际上微生物生态功能多样性研究的最典型和最权威方法之一。
每块96孔ECO板上有3组平行,每组31种碳源,3个阴性对照,加入同一样品后,可以获得3组平行数据。微生物群落对这31种碳源的特征性利用称作该微生物群落的代谢指纹图谱。从单个的微平板上的指纹图谱就可以获得大量的代谢信息。可用于纯种及混合菌群(如土壤样品)微生物功能多样性研究。 31种碳源可分为六大类,具体如下: 单糖\糖苷\聚合糖类 B2, D-木糖 H1, a-D-乳糖 A2, ß-甲基D-葡萄糖苷 G2,葡萄糖-1-***酸盐 E1, a-环状糊精 F1, 肝糖 G1, D-纤维二糖 氨基酸类, A4,L-精氨酸, B4,L-天冬酰胺酸 C4 L-***基丙氨酸 D4, L-丝氨酸 E4,L-苏氨酸 F4,甘氨酰-L-谷氨酸 酯类 B1, 丙***酸甲酯 C1, 吐温40 D1, 吐温80 A3, D-半乳糖酸γ内酯 醇类 C2, I-赤藻糖醇 D2, D-甘露醇 H2, D,L-a-甘油 胺类 G4, ***乙基胺 H4, 腐胺 E2, N-乙酰基-D-葡萄胺 酸类 B3,D-半乳糖醛酸 F2 D-氨基葡萄糖酸 C3, 2-羟***甲酸 D3, 4-羟基***甲酸 E3,r-羟基丁酸 F3, 衣康酸 G3,a-丁***酸 H3, D-苹果酸 样品简单处理后(过滤或离心),取上清液加到ECO板上培养多至5天时间,每隔一定时间用酶标仪在590nm和750nm下测定OD值,OD值的大小反应样品微生物利用某一碳源底物的能力,将OD值导出后进行分析,如AWCD值(平均吸光度)、丰富度、单孔的动力学分析、不同类碳源的比较等,并可用聚类软件对不同样品进行聚类分析。 值得一提的是ECO板不仅测定可培养微生物,还可测定部分不可培养微生物,只要微生物能利用碳源进行代谢(并不增殖),ECO板上显色体系均可检测出来。 最常见的应用是利用ECO板分析环境因素、根系、叶面、肥料、***对土壤微生物菌群的生态功能多样性的影响,比较不同样品间或同一样品不同时间的功能多样性差异,比较环境修复前后微生物群落的功能多样性差异,等等。 BIOLOG 鉴定板与培养基 Microplate Panels and Media GEN II & GEN III MicroPlate Consumables | | · Sold in increments of 10 plates/box · Consumable products are NON-RETURNABLE Catalog Number | Description | 1030 | GEN III MicroPlate (for both Gram-negative & Gram-positive bacteria) | 1011 | GN2 MicroPlate (GEN II for Gram-negative bacteria) | 1014 | GP2 MicroPlate (GEN II for Gram-positive bacteria) | 1007 | AN MicroPlate (GEN II for Anaerobic bacteria) | 1005 | YT MicroPlate (GEN II for Yeasts) | 1006 | FF MicroPlate (GEN II for Filamentous Fungi) | Special Purpose MicroPlates · Sold in increments of 10 plates/box · Consumable products are NON-RETURNABLE Catalog Number | Description | 1013 | MT2 MicroPlate (for metabolic studies) | 1511 | SF-N2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-negative microbes) | 1514 | SF-P2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-positive microbes) | 1506 | ECO MicroPlate - 3 sets of the same 31 carbon sources in a 96 well Biolog MicroPlate. Used for characterization / community analysis) | Biolog Prepared Plated Media · Sold in increments of 15 plates/pack Catalog Number | Description | 71102 | BUG (Biolog Universal Growth) Agar w/ 5% sheep blood | 71103 | BUG (Biolog Universal Growth) Agar w/ 0.25% maltose1 | 71005 | BUY (Biolog Universal Yeast) Agar1 | Other Prepared Plated Media · Sold in increments of 10 plates/pack Catalog Number | Description | Bio-M1040 | Tryptic Soy Agar (TSA) | Bio-M1006 | Tryptic Soy Agar (TSA) w/ 5% sheep blood | Bio-M1012 | Chocolate Agar, enriched | 71007 | BUA (Biolog Universal Anaerobe) Agar w/5% sheep blood | Bio-M1065 | R2A Agar | 71106 | 2% Malt Extract Agar | Biolog Dehydrated Media Catalog Number | Description | 70101 | BUG (Biolog Universal Growth) Agar - 500 g jar (approx. 435 plates) | 70007 | BUA (Biolog Universal Anaerobe) Agar | 70005 | BUY (Biolog Universal Yeast) Agar - 500 g jar (approx. 415 plates) | GEN III Starter Kits · Kits containing the consumables a new user would need to begin using the OmniLog System for bacterial indentifications Catalog Number | Description | 39993A-O | 20 GEN III MicroPlates · 1 box of pipette tips (Ovation) · 1 CASE OF reservoirs · 1 package OF Inoculatorz (100 units individually packaged) · 20 tubes OF IF-A · 20 tubes OF IF-B | 39993A-M | 20 GEN III MicroPlates · 1 box of pipette tips (Matrix) · 1 CASE OF reservoirs · 1 package OF Inoculatorz (100 units individually packaged) · 20 tubes OF IF-A · 20 tubes OF IF-B | 39993B | 30 BUG+blood plated media for GEN III | | |