17-1082-01 | PHENYL SEPHAROSE HIGH PERF 75ML | 17-1087-01 | SP SEPHAROSE HIGH PERF 75 ML | 17-1273-01 | SOURCE 30S, 50 ML | 17-1275-01 | SOURCE 30Q, 50 ML | 17-1287-01 | ANX SEPH 4 FF HIGH SUB 500ML | 17-1287-10 | ANX SEPHAROSE FF HIGHSUB 25 ML | 17-5072-01 | Q SEPHAROSE XL, 300 ML | 17-5073-01 | SP SEPHAROSE XL, 300 ML | 17-5316-02 | Capto Q 100 ml | 17-5316-10 | CAPTO Q, 25 ML | 17-5317-02 | Capto MMC 100ml | 17-5317-10 | CAPTO MMC, 25ML | 17-5432-01 | Butyl Sepharose HP, 25 ml | 17-5432-02 | Butyl Sepharose HP, 200ml | 17-5437-01 | Q SEPHAROSE XL VIRUS LIC 300ML | 17-5437-10 | Q SEPHAROSE XL VIRUS LIC 25 ML | 17-5441-01 | CAPTO S, 100 ML | 17-5441-10 | CAPTO S, 25 ML | 17-5443-01 | CAPTO DEAE, 100 ML | 17-5443-10 | CAPTO DEAE, 25 ML | 17-5444-10 | CAPTO adhere 25 ML | HiLoad™ Phenyl Sepharose™ HP Columns and Phenyl Sepharose™ High Performance Lab Packs - Aromatic HIC medium for intermediate purification and polishing
- Selectivity similar to Phenyl Sepharose 6 Fast Flow (low sub) but higher resolution due to the smaller 34 μm bead size
HiLoad™ Phenyl Sepharose™ HP Columns and Phenyl Sepharose™ High Performance Lab PacksTechnical Information
TECHNICAL SPECIFICATIONS | Medium | Phenyl Sepharose™ High Performance | Ligand density | 25 μmol/mg medium | Binding capacity | 20 mg IgG/ml medium 24 mg HSA/ml medium | Average particle size | 34 μm | Rec. linear flow rate | 30-150 cm/h | Max. pressure | 5 bar (73 psi, 0.5 MPa) | Chemical stability | Stable in common buffers, chaotropic agents, detergents, and polar organic solvents. | pH stability | 3-12 (working and long term) 2-14 (short term) | Storage | 20% ethanol | Storage temperature | 4°C to 30°C | Prepacked HiLoad™ Columns | | Bed volume | | HiLoad™ 16/10 | 20-22 ml | HiLoad™ 26/10 | 53-58 ml | Bed dimensions | | HiLoad™ 16/10 | 1.6 × 10 cm | HiLoad™ 26/10 | 2.6 × 10 cm | Efficiency | > 12 000 m-1 | Rec. flow rate | Up to 150 cm/h | Max. back pressure over the packed bed during operation | 3 bar (42 psi, 0.3 MPa) | HiLoad™ column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) | Storage | 20% ethanol | Storage temperature | 4°C to 30°C | Separation of conformational isomers of a2-macroglobulin on Phenyl Sepharose™ High Performance
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