| 17-1287-01 | ANX SEPH 4 FF HIGH SUB 500ML | | 17-1287-10 | ANX SEPHAROSE FF HIGHSUB 25 ML | | 17-5072-01 | Q SEPHAROSE XL, 300 ML | | 17-5073-01 | SP SEPHAROSE XL, 300 ML | | 17-5316-02 | Capto Q 100 ml | | 17-5316-10 | CAPTO Q, 25 ML | | 17-5317-02 | Capto MMC 100ml | | 17-5317-10 | CAPTO MMC, 25ML | | 17-5432-01 | Butyl Sepharose HP, 25 ml | | 17-5432-02 | Butyl Sepharose HP, 200ml | | 17-5437-01 | Q SEPHAROSE XL VIRUS LIC 300ML | | 17-5437-10 | Q SEPHAROSE XL VIRUS LIC 25 ML | | 17-5441-01 | CAPTO S, 100 ML | | 17-5441-10 | CAPTO S, 25 ML | | 17-5443-01 | CAPTO DEAE, 100 ML | | 17-5443-10 | CAPTO DEAE, 25 ML | | 17-5444-10 | CAPTO adhere 25 ML | IEX Sepharose™ Fast Flow - For preparative purifications, even when purifying crude samples.
- Available with strong (Q and SP) and weak (DEAE, CM, and ANX) anion and cation exchangers, in bulk quantities and in prepacked HiTrap™ and HiPrep™ columns, as well as HiTrap™ IEX Selection Kit.
- For separation of proteins of all pI values with Q and SP having a high dynamic capacity for proteins over the entire pH range.
- ANX has a different selectivity compared to DEAE, and is applicable for separation of high molecular weight proteins.
- Easy scale-up from HiTrap™ scale to laboratory scale to process scale.
IEX Sepharose™ Fast FlowTechnical Information
| TECHNICAL SPECIFICATIONS | | Ion exchanger type | | | Q Sepharose™ FF | Quaternary ammonium strong anion | | DEAE Sepharose™ FF | Diethylaminoethyl weak anion | | ANX Sepharose™ 4 FF | Diethylaminopropyl weak anion | | SP Sepharose™ FF | Sulfopropyl strong cation | | CM Sepharose™ FF | Carboxymethyl weak cation | | Ionic capacity | | | Q Sepharose™ FF | 0.18-0.25 mmol (Cl-)/ml | | DEAE Sepharose™ FF | 0.11-0.16 mmol (Cl-)/ml | | ANX Sepharose™ 4 FF | 0.13-0.18 mmol (Cl-)/ml | | SP Sepharose™ FF | 0.18-0.25 mmol (H+)/ml | | CM Sepharose™ FF | 0.09-0.13 mmol (H+)/ml | | Dynamic capacity | | | Q Sepharose™ FF | 120 mg HSA/ml medium | | DEAE Sepharose™ FF | 110 mg HSA/ml medium | | ANX Sepharose™ 4 FF | 5 mg thyroglobulin/ml medium | | SP Sepharose™ FF | 70 mg RNase/ml medium | | CM Sepharose™ FF | 50 mg RNase/ml medium | | Pressure/flow spec. | | | Q Sepharose™ FF | 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm | | DEAE Sepharose™ FF | 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm | | ANX Sepharose™ 4 FF | min 200 cm/h, 100 kPa, XK50/60 column, bed height 25 cm | | SP Sepharose™ FF | 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm | | CM Sepharose™ FF | 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm | | Average particle size | 90 μm (45-165 μm) | | Matrix | | | Sepharose™ FF | Highly cross-linked agarose, 6% | | Sepharose™ 4 FF | Highly cross-linked agarose, 4% | | pH stability | | | Q Sepharose™ FF | 1-14 (short term), 2-12 (long term) | | DEAE Sepharose™ FF | 1-14 (short term), 2-13 (long term) | | ANX Sepharose™ 4 FF | 2-14 (short term), 3-13 (long term) | | SP Sepharose™ FF | 3-14 (short term), 4-13 (long term) | | CM Sepharose™ FF | 2-14 (short term), 4-13 (long term) | Chemical stability
| Stable in all common aqueous buffers:
8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH*, and 1 M acetic acid*. | | Storage | 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) | | Storage temperature | 4°C to 30°C | | *1 M NaOH and acetic acid should be used only for cleaning purposes. | http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=8477 |