Yeast Protein Extraction Buffer Kit

 

Yeast Protein Extraction Buffer Kit is a mild detergent-based cell lysis method developed for total extraction of soluble proteins from yeast cells. This kit eliminates the need for glass beads, a common method of mechanical lysis for yeast cells, and is a proprietary improvement on the Zymolyase based method for spheroplast preparation and extraction of soluble proteins from yeast cells. After cell lysis, Yeast Protein Extraction Buffer is added to the yeast pellets, referred to as spheroplasts, to extract biologically active proteins. The kit is compatible with any downstream application including enzymes assays, chromatography procedures, and gel electrophoresis applications, and is suitable for preparation of approximately 10 ml yeast cell pellets suspension as single or as multiple smaller preps.

Yeast Protein Extraction Buffer Kit — Technical Specifications

Complete Packsize	1 Piece
Includes	100 ml Yeast protein extraction buffer 15 ml Yeast sSuspension buffer 2 × 0.5 ml Longlife Zymolyase (1500 U/ml)
Storage Conditions	Longlife Zymolyase below -18°C and other kit components at 2–8°C
Wetted Part	No
Guidance	The kit is used for extraction of proteins from yeast cells. Expected shelf life: Stable for 1 year from manufacturing date when stored and used as recommended