28-9412-79 Mammalian Cell Lysis Buffer

28-9435-22 Extraction Buffer Trial Kit

28-9435-23 2-D Protein Extraction BufferI

28-9435-24 2-D ProteinExtraction BufferII

28-9435-25 2-DProteinExtraction BufferIII

28-9435-26 2-DProteinExtraction Buffer IV

28-9435-27 2-DProteinExtractionBufferV

28-9435-28 2-DProteinExtractionBufferVI

28-9440-06 Protein A Mag Sepharose 1x500uL

28-9440-08 Protein G Mag Sepharose 1 x 500UL

28-9440-09 NHS Mag Sepharose 1x500ul

28-9440-10 TiO2 Mag Sepharose 1x500ul

28-9440-45 Yeast Buffer Kit

28-9466-03 HiTrap Albumin IgG Depl,2x1 ml

28-9480-20 Albumin IgG Depletion SpinTrap

28-9489-64 Mag Rack 6

28-9513-77 TiO2 Mag Sepharose 4x500ul

28-9513-78 Protein A Mag Sepharose 4x500u

28-9513-79 Protein G Mag Sepharose 4x500u

28-9513-80 NHS Mag Sepharose 4x500ul

Albumin & IgG Depletion SpinTrap

Albumin & IgG Depletion SpinTrap are prepacked columns for the depletion of albumin and IgG from human serum and plasma. They are prepacked with high performance SepharoseTM based media with an affinity for human serum albumin (HSA) and IgG

Albumin & IgG Depletion SpinTrap is a prepacked column for the depletion of albumin and IgG from human serum or plasma.
Albumin & IgG Depletion SpinTrap handles smaller sample volumes (~50 μl) using a tabletop centrifuge.
Columns are prepacked with high performance Sepharose based media with an affinity for human serum albumin (HSA) and IgG.
High depletion capacity, removes > 95% HSA and > 90% IgG, with high reproducibility.
Simple and fast procedure.
Removal of albumin and IgG allows a higher load of less abundant proteins to be included in the analysis and detection of more proteins.

Albumin & IgG Depletion SpinTrap and HiTrap Albumin & IgG Depletion are prepacked columns for the depletion of albumin and IgG from plasma and serum samples. Albumin and IgG are the most abundant plasma proteins, and they tend to obscure the signals of less abundant proteins. Depletion of the two proteins removes more than 60% of the total protein content in human plasma, allowing a higher load of less abundant proteins to be included in the analysis and detection of more proteins. These columns are members of the Trap platform and address the need for flexible, small-scale preparation of protein samples prior to downstream analyses such as 1-D or 2-D gel electrophoresis and mass spectrometry.

Albumin & IgG Depletion SpinTrap — Technical Specifications

Complete Packsize 10

Bed Volume 370 µl

Storage Conditions 2 to 8°C, in 20% Ethanol

Wetted Part No

Guidance Prepacked columns for the depletion of albumin and plasma.

For larger scale please use HiTrap Albumin & IgG Depletion column

Media

Ligand Recombinant Protein G fragment and recombinant protein binding HSA

Average Particle Size 34 μm

Sample Loading Volume 150 μl undiluted human plasma

pH stability Working Range 3-9

pH stability Cleaning 2-9

Flow rate <4 ml/min

Storage Conditions 20% Ethanol at 4 to 8°C