17-0717-01	HITRAP NHS-ACTIV HP, 1 X 5 ML
17-0920-03	HITRAP IMAC HP, 5 X 1 ML
17-0920-05	HITRAP IMAC HP, 5 X 5 ML
17-0921-02	HITRAP IMAC FF, 5 X 1ML
17-0921-04	HITRAP IMAC FF, 5 X 5 ML
17-0978-13	HITRAP BUTYL-S FF, 5 X 1ML
17-0978-14	HITRAP BUTYL-S FF, 5 X 5ML
17-1128-01	MABTRAP KIT
17-1151-01	HITRAP SP HP, 5 X 1 ML
17-1152-01	HITRAP SP HP, 5X5 ML
17-1153-01	HITRAP Q HP, 5 X 1 ML
17-1154-01	HITRAP Q HP 5X5 ML
17-1351-01	HITRAP PHENYL HP, 5 X 1ML
17-1353-01	HITRAP PHENYL FF (LS), 5X1ML
17-1355-01	HITRAP PHENYL FF (HS),5 X 1ML
17-1357-01	HITRAP BUTYL FF, 5 X 1 ML
17-1359-01	HITRAP OCTYL FF, 5 X 1 ML
17-1408-01	HITRAP DESALTING, 5X5ML
17-5053-01	HITRAP Q FF, 5 X 1 ML
17-5054-01	HITRAP SP FF, 5 X 1 ML
17-5055-01	HITRAP DEAE FF 5 X 1 ML
17-5056-01	HITRAP CM FF, 5 X 1 ML
17-5079-01	HITRAP RPROTEIN A FF, 5X1ML
17-5079-02	HITRAP RPROTEIN A FF, 2 X 1 ML
17-5080-01	HITRAP RPROTEIN A FF, 1X5ML
17-5080-02	HITRAP RPROTEIN A FF, 5 X 5 ML
17-5110-01	HITRAP IGM PURIFICATION 5X1ML
17-5111-01	HITRAP IGY PURIFICATION 1X5 ML
17-5112-01	HITRAP STREPTAVIDIN HP5X1 ML
17-5130-01	GSTRAP FF, 5X1ML
17-5130-02	GSTRAP FF, 2X1ML
17-5130-05	GSTRAP FF, 100 X 1 ML
17-5131-01	GSTRAP FF, 1X5ML
17-5131-02	GSTRAP FF 5 X 5 ML
17-5131-05	GSTRAP FF, 100 X 5 ML
17-5143-01	HITRAP BENZAMIDINE FF 5 X 1ML
17-5143-02	HITRAP BENZAMIDINE FF 2 X 1 ML
17-5144-01	HITRAP BENZAMIDINE FF 1 X 5 ML
17-5154-01	HITRAP DEAE FF 5 X 5 ML
17-5155-01	HITRAP CM FF, 5 X 5 ML
17-5156-01	HITRAP Q FF, 5 X 5 ML
17-5157-01	HITRAP SP FF, 5 X 5 ML
17-5158-01	HITRAP Q XL, 5 X 1 ML
17-5159-01	HITRAP Q XL, 5 X 5 ML
17-5160-01	HITRAP SP XL, 5 X 1 ML
17-5161-01	HITRAP SP XL, 5 X 5 ML
17-5162-01	HITRAP ANX FF (HIGHSUB)5 X 1ML
17-5163-01	HITRAP ANX FF(HIGHSUB) 5 X 5ML
17-5193-01	HITRAP PHENYL FF (HS), 5 X 5ML
17-5194-01	HITRAP PHENYL FF (LS), 5X 5ML
17-5195-01	HITRAP PHENYL HP, 5 X 5 ML
17-5196-01	HITRAP OCTYL FF, 5 X 5 ML
17-5197-01	HITRAP BUTYL FF, 5 X 5 ML
17-5247-01	HISTRAP HP 5 X 1 ML
17-5247-05	HISTRAP HP 100 X 1 ML
17-5248-01	HISTRAP HP 1 X 5 ML
17-5248-02	HISTRAP HP 5 X 5 ML
17-5248-05	HISTRAP HP 100 X 5 ML
17-5255-01	HISTRAP FF 5 X 5 ML
17-5255-02	HISTRAP FF 100 X 5 ML
17-5281-01	GSTRAP HP 5 X 1 ML
17-5281-05	GSTRAP HP 100 X 1 ML
17-5282-01	GSTRAP HP 1 X 5 ML
17-5282-02	GSTRAP HP 5 X 5 ML
17-5282-05	GSTRAP HP, 100 X 5 ML
17-5286-01	HISTRAP FF CRUDE 5X5 ML
17-5286-02	HISTRAP FF CRUDE 100X5 ML
17-5319-01	HISTRAP FF 5 X 1 ML
17-5319-02	HISTRAP FF 100 X 1 ML
17-5441-22	HITRAP CAPTO S, 5X1 ML
17-5441-23	HITRAP CAPTO S, 5X5 ML
17-6002-33	HITRAP IEX SELECTION KIT
18-1142-75	REC PROTEIN PURIF HANDBOOK
28-4010-81	HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77	HISTRAP FF CRUDE KIT
28-4017-45	GSTRAP 4B, 5 X 1 ML
28-4017-46	GSTRAP 4B, 100 X 1 ML
28-4017-47	GSTRAP 4B, 1 X 5 ML
28-4017-48	GSTRAP 4B, 5 X 5 ML
28-4017-49	GSTRAP 4B, 100 X 5 ML
28-4058-44	HiTrap Capto Adhere 5x1ml
28-4058-46	5x5 ml Hitrap Capto adhere
28-4082-53	HITRAP MABSELECT, 5 X 1 ML
28-4082-55	HITRAP MABSELECT, 1 X 5 ML
28-4082-56	HITRAP MABSELECT, 5 X 5 ML
28-4082-58	HITRAP MABSELECT XTRA, 5X1ML
28-4082-60	HITRAP MABSELECT XTRA, 1X5ML
28-4082-61	HITRAP MABSELECT XTRA, 5X5ML
28-4110-01	HiTrap Butyl HP, 5x1ml
28-4110-05	HiTrap Butyl HP, 5 x 5ml
28-4110-07	HiTrap HIC Selection Kit, 7x1

HiTrap™ NHS-activated HP Columns

• Prepacked with NHS-activated Sepharose™ High Performance for coupling ligand via primary amines at near neutral pH.
• Simple coupling, carried out at close to neutral pH.
• Delivered in 100% isopropanol to protect the active groups.

• HiTrap™ NHS-activated HP Columns

  Technical Information

  
  
  Lane 1: Desorbed material, reduced
  Lane 2: Low Molecular Weight (LMW) calibration kit, reduced
  Lane 1: Desorbed material, non-reduced
  Lane 2: Low Molecular Weight (LMW) calibration kit, reduced
  

   

  The use of NHS-activated Sepharose™ High Performance is illustrated by the purification of anti-mouse Fc-IgG from sheep antiserum after coupling mouse IgG to HiTrap™ NHS-activated. The contents of the different fractions were analysed by SDS-PAGE using PhastSystem™ and Goomassie staining.
  

   

  TECHNICAL SPECIFICATIONS
  Group to be coupled	-NH2
  Coupling conditions	pH 6.5-9 at 4°C to 25°C
  Matrix	Highly cross-linked agarose, 6%
  Substitution	10 μmol NHS/ml medium
  Spacer arm	6-aminocaproic acid, 10-atom
  pH stability*	3-12
  Average particle size	34 μm
  Storage	100% isopropanol
  Storage temperature	4°C to 8°C
  * Ligand dependent.

   

  For column specifications, see Prepacked HiTrap™ Affinity Columns.

   

  
  Purification of anti-mouse Fc-IgG from sheep antiserum.