英文名称： HILOAD 16/10 Q SEPHAROSE HP
| 17-1064-01 | HILOAD 16/10 Q SEPHAROSE HP |
| 17-1066-01 | HILOAD 26/10 Q SEPHAROSE HP |
| 17-1068-01 | HILOAD 16/60 SUPERDEX 75 PG |
| 17-1069-01 | HILOAD 16/60 SUPERDEX 200 PG |
| 17-1070-01 | HILOAD 26/60 SUPERDEX 75 PG |
| 17-1071-01 | HILOAD 26/60 SUPERDEX 200 PG |
| 17-1085-01 | HILOAD 16/10 PHENYL SEPHAROSE |
| 17-1086-01 | HILOAD 26/10 PHENYL SEPHAROSE |
| 17-1137-01 | HILOAD 16/10 SP SEPHAROSE HP |
| 17-1138-01 | HILOAD 26/10 SP SEPHAROSE HP |
| 17-1139-01 | HILOAD 16/60 SUPERDEX 30 PG |
| 17-1140-01 | HILOAD 26/60 SUPERDEX 30 PG |
HiLoad™ IEX HP Columns and IEX Sepharose™ High Performance Lab Packs
- High-performance preparative separations where high efficiency and high resolution are desired.
- Highly cross-linked agarose matrix provides excellent chemical and physical stability.
- Available as strong anion exchangers (Q Sepharose HP) and strong cation exchangers (SP Sepharose HP), analogous to Mono Q™ and S, and Q and SP Sepharose™ Fast Flow.
- Easy to use. For optimal performance and reproducibility, Sepharose™ High Performance is also available in prepacked HiTrap™ columns.
HiLoad™ IEX HP Columns and IEX Sepharose™ High Performance Lab Packs
Technical Information
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Sepharose™ High Performance ion exchangers provide highly efficient, high-resolution preparative scale separations.
| TECHNICAL SPECIFICATIONS | |
| Ion exchanger type | |
| Q Sepharose™ HP | Quaternary ammonium strong anion exchanger |
| SP Sepharose™ HP | Sulfopropyl strong cation exchanger |
| Charged groups | |
| SP Sepharose™ HP | - SO3- |
| Q Sepharose™ HP | - N+(CH3)3 |
| Ionic capacity | |
| Q Sepharose™ HP | 0.14-0.20 mmol Cl-/ml |
| SP Sepharose™ HP | 0.15-0.20 mmol H+/ml |
| Dynamic capacity | |
| Q Sepharose™ HP | 70 mg BSA/ml medium |
| SP Sepharose™ HP | 55 mg ribonuclease/ml medium |
| Rec. flow velocity | 30-150 cm/h |
| Max. pressure over the packed bed in a HiLoad column during operation | 3 bar (42 psi, 0.3 MPa) |
| HiLoad™ column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) |
| Average particle size | 34 μm |
| Exclusion limit (Mr) | » 4 ×106 globular protein |
| Matrix | cross-linked agarose, 6% |
| pH stability | |
| Q Sepharose™ HP | 2-12 (working and long term), 1-14 (short term) |
| SP Sepharose™ HP | 4-13 (working and long term), 3-14 (short term) |
| Chemical stability | Stable in all commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid* |
| Storage | 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
| Prepacked HiLoad™ Columns | |
| Bed volume | |
| HiLoad™ 16/10 | 20 ml |
| HiLoad™ 26/10 | 53 ml |
| Bed dimensions | |
| HiLoad™ 16/10 | 16 mm × 100 mm |
| HiLoad™ 26/10 | 26 mm × 100 mm |
| Efficiency | > 12 000 m-1 |