28-9365-50

GSTPrep FF 16/10

1 ×20 ml

GSTPrep™ FF 16/10 Column - Easy Scale-up Purification

Technical Information

GSTPrep™ FF 16/10 for fast and easy scale-up purification of GST-tagged recombinant proteins.

 

GSTPrep™ FF 16/10 are 20-ml HiPrep™ columns prepacked with Glutathione Sepharose™ 4 Fast Flow for scale-up purification of recombinant GST-tagged proteins, other glutathione S-transferases, and glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow in combination with HiPrep™ column hardware provides excellent flow properties.

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165074

 

TECHNICAL SPECIFICATIONS
Medium	Glutathione Sepharose™ 4 Fast Flow
Column volume	20 ml
Column dimensions	1.6 × 10 cm
Recommended flow rate*	1-10 ml/min (30-300 cm/h)
Max. flow rate*	10 ml/min (300 cm/h)
Max. pressure over  the packed bed during operation*	1.5 bar (0.15 MPa, 22 psi)
Column hardware pressure limit	5 bar (0.5 MPa, 73 psi)
Storage	20% ethanol
Storage temperature	4°C to 30°C
* H2O at room temperature. Note: Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider, especially during sample loading and elution.