28-9412-79 Mammalian Cell Lysis Buffer

28-9435-22 Extraction Buffer Trial Kit

28-9435-23 2-D Protein Extraction BufferI

28-9435-24 2-D ProteinExtraction BufferII

28-9435-25 2-DProteinExtraction BufferIII

28-9435-26 2-DProteinExtraction Buffer IV

28-9435-27 2-DProteinExtractionBufferV

28-9435-28 2-DProteinExtractionBufferVI

28-9440-06 Protein A Mag Sepharose 1x500uL

28-9440-08 Protein G Mag Sepharose 1 x 50

28-9440-09 NHS Mag Sepharose 1x500ul

28-9440-10 TiO2 Mag Sepharose 1x500ul

28-9440-45 Yeast Buffer Kit

28-9466-03 HiTrap Albumin IgG Depl,2x1 ml

28-9480-20 Albumin IgG Depletion SpinTrap

28-9489-64 Mag Rack 6

28-9513-77 TiO2 Mag Sepharose 4x500ul

28-9513-78 Protein A Mag Sepharose 4x500u

28-9513-79 Protein G Mag Sepharose 4x500u

28-9513-80 NHS Mag Sepharose 4x500ul

Protein A Mag Sepharose

Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose with native protein A as ligand.

Visible and dense Sepharose based magnetic beads with protein A ligand, easy to spot and collect bound target protein
Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed
Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A as ligand. Protein A has high affinity for the Fc region of IgG antibodies from a broad variety of species

There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis

Protein A Mag Sepharose combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel

Protein A Mag Sepharose — Technical Specifications

Application Designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications
Used for biomarker reseach

Matrix Paramagnetic, spherical, highly cross-linked agarose particles

Ligand Native protein A

Binding Capacity 8 mg human IgG/ml gel - 17 mg human IgG/ml gel

Operating Temp. Room temperature

Storage Conditions 4 - 8°C, 20% Ethanol, 20% medium slurry

Stability Stable