28-4017-45 GSTRAP 4B, 5 X 1 ML

28-4017-46 GSTRAP 4B, 100 X 1 ML

28-4017-47 GSTRAP 4B, 1 X 5 ML

28-4017-48 GSTRAP 4B, 5 X 5 ML

28-4017-49 GSTRAP 4B, 100 X 5 ML

28-4058-44 HiTrap Capto Adhere 5x1ml

28-4058-46 5x5 ml Hitrap Capto adhere

28-4082-53 HITRAP MABSELECT, 5 X 1 ML

28-4082-55 HITRAP MABSELECT, 1 X 5 ML

28-4082-56 HITRAP MABSELECT, 5 X 5 ML

28-4082-58 HITRAP MABSELECT XTRA, 5X1ML

28-4082-60 HITRAP MABSELECT XTRA, 1X5ML

28-4082-61 HITRAP MABSELECT XTRA, 5X5ML

28-4110-01 HiTrap Butyl HP, 5x1ml

28-4110-05 HiTrap Butyl HP, 5 x 5ml

28-4110-07 HiTrap HIC Selection Kit, 7x1

28-9075-46 StrepTrap HP 5 x 1mL

28-9075-47 StrepTrap HP 1 x 5mL

28-9075-48 StrepTrap HP 5 x 5mL

28-9165-37 HiTrap Capto DEAE, 5X1ML

28-9165-40 HiTrap Capto DEAE, 5X5ML

28-9187-78 MBPTrap 5 x 1mL

28-9187-79 MBPTrap 1 x 5 mL

28-9187-80 MBPTrap 5 x 5mL

28-9343-88 HiTrap Capto IEX Selection Kit

28-9520-85 HiTrap Con A 4B 5 x 1 ml

28-9520-96 HiTrap Con A 4B 5 x 5 ml

Glutathione Sepharose™ 4B Media/GSTrap 4B Columns - Easy Purification

Fast and simple purification of GST*-tagged proteins.
GSTrap™ 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ 4B.
GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTAdesign

Glutathione Sepharose™ 4B Media/GSTrap 4B Columns - Easy Purification

Technical Information

GSTrap 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose 4B for fast purification of GST-tagged proteins and scale-up.

GSTrap™ 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.

For an introduction to Glutathione S-transferase (GST) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System.

Columns: Capture GSTrap 4B 1 ml
Polishing HiLoad 16/60 Superdex 200 pg

Sample: Cell extract from E. coli expressing GST-hippocalcin (M_r 45 000)
after lysis with CelLytic Express

AC binding/wash buffer: 10 mM sodium phosphate, 140 mM, pH 7.4

AC elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0

GF buffer: 50 mM phosphate buffer, 150 mM NaCl, pH 7.2

System: ÄKTAprime plus

1 Low molecular weight markers
2 Start material (diluted 1:20)
3 Eluted pool GSTrap 4B
4 Eluted pool GSTrap 4B (diluted 1:50)
5 Peak 1, HiLoad 16/60 Superdex 200 pg
6 Peak 2, HiLoad 16/60 Superdex 200 pg

TECHNICAL SPECIFICATIONS

Ligand Glutathione

Ligand coupling method Epoxy activation

Dynamic binding capacity* > 5 mg horse liver GST/ml medium

Matrix Agarose, 4%

Average particle size 90 μm

pH stability 4-13

Storage 20% ethanol

Storage temperature 4°C to 30°C

HiTrap prepacked columns

Column volume 1 ml and 5 ml

Column dimensions 0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml)

Recommended flow rate^† 0.2-1 ml/min (1 ml); 0.5-5 ml/min (5 ml)

Max. pressure^† 3 bar (0.3 MPa)

* Binding of GST to glutathione is flow-rate dependent and lower flow rates often iincrease the binding capacity. This is an important consideration, especially during sample loading and elution.
^† H₂O at 25°C.

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