28-9365-45 | HiPrep Phenyl FF (high sub) 16 | 1 ×20 ml |
28-9365-46 | HiPrep Phenyl FF (low sub) 16/ | 1 ×20 ml |
28-9365-47 | HiPrep Butyl FF 16/10 | 1 ×20 ml |
28-9365-48 | HiPrep Octyl FF 16/10 | 1 ×20 ml |
28-9365-49 | HiPrep Heparin FF 16/10 | 1 ×20 ml |
HiPrep™ Phenyl FF (high sub) 16/10, HiPrep™ Phenyl FF (low sub) 16/10, HiPrep™ Butyl FF 16/10 and HiPrep™ Octyl FF 16/10 Columns
Technical Information
TECHNICAL SPECIFICATIONS |
Media | Phenyl Sepharose™ 6 Fast Flow (high sub) Phenyl Sepharose™ 6 Fast Flow (low sub) Butyl Sepharose™ 4 Fast Flow Octyl Sepharose™ 4 Fast Flow |
Column dimensions | 1.6 × 10 cm |
Bed volume | 20 ml |
Recommended flow rate* | 2-10 ml/min (60-300 cm/h) |
Max. flow rate* | 10 ml/min (300 cm/h) |
Max. pressure over the packed bed during operation | 1.5 bar (22 psi, 0.15 MPa) |
Column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) |
Storage | 20% ethanol |
Storage temperature | 4°C to 30°C |
* H2O at 25°C. | |
Partial purification of alkaline phosphatase from E. coli on HiPrep™ Phenyl FF (high sub) 16/10.
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