11-0032-73	HITRAP CAPTO MMC, 5X1ML
11-0032-75	HITRAP CAPTO MMC, 5X5ML
11-0034-93	HITRAP MABSELECT SURE, 5X1ML
11-0034-94	HITRAP MABSELECT SURE, 1X5ML
11-0034-95	HITRAP MABSELECT SURE, 5X5ML
17-0402-01	HITRAP PROTEIN A HP, 5 X 1 ML
17-0402-03	HITRAP PROTEIN A HP,2 X 1 ML
17-0403-01	HITRAP PROTEIN A HP,1 X 5 ML
17-0403-03	HITRAP PROTEIN A HP, 5 X 5 ML
17-0404-01	HITRAP PROTEIN G HP, 5 X 1 ML
17-0404-03	HITRAP PROTEIN G HP, 2 X 1 ML
17-0405-01	HITRAP PROTEIN G HP,1 X 5 ML
17-0405-03	HITRAP PROTEIN G HP, 5 X 5 ML
17-0406-01	HITRAP HEPARIN HP, 5 X 1 ML
17-0407-01	HITRAP HEPARIN HP,1 X 5 ML
17-0407-03	HiTrap Heparin HP 5x5ml
17-0408-01	HITRAP CHELATING HP, 5 X 1 ML
17-0409-01	HITRAP CHELATING HP, 1 X 5 ML
17-0409-03	HITRAP CHELATING HP 5 X 5 ML
17-0409-05	HITRAP CHELATING HP 100 X 5 ML
17-0412-01	HITRAP BLUE HP, 5 X 1 ML
17-0413-01	HITRAP BLUE HP, 1 X 5 ML
17-0716-01	HITRAP NHS-ACTIV HP, 5 X 1ML
17-0716-30	HiTrap NHS-activated HP, 180 x
17-0717-01	HITRAP NHS-ACTIV HP, 1 X 5 ML
17-0920-03	HITRAP IMAC HP, 5 X 1 ML
17-0920-05	HITRAP IMAC HP, 5 X 5 ML
17-0921-02	HITRAP IMAC FF, 5 X 1ML
17-0921-04	HITRAP IMAC FF, 5 X 5 ML
17-0978-13	HITRAP BUTYL-S FF, 5 X 1ML
17-0978-14	HITRAP BUTYL-S FF, 5 X 5ML

Capto MMC Media/HiTrap Capto MMC

• High dynamic binding capacity at high conductivity.
• High volume throughput.
• New selectivity allows binding in the presence of salt.
• Prepacked HiTrap™ columns for easy screening and convenient process development.

Capto MMC Media/HiTrap Capto MMC

Technical Information

Capto MMC is a salt-tolerant multimodal cation exchanger for process-scale applications. Capto MMC gives increased productivity and reduced cost.

 

Capto™ MMC is a multimodal cation exchanger. The multimodal functionality gives a different selectivity compared with traditional ion exchangers. This includes binding of proteins regardless of ionic strength of the loading material (see Figure below). This means that the medium may be used for direct load of clarified feedstocks, without prior dilution to reduce the conductivity of the starting material. The novel selectivity of Capto MMC can also be used to solve specific purification problems, at high as well as at low ionic strength.

 

HiTrap™ Capto MMC are prepacked 1-ml and 5-ml columns for screening of selectivity, binding and elution conditions, and method scouting. To reduce tedious sample preparation, the top filter in HiTrap Capto MMC has been optimized for loading sonicated unclarified cell lysate directly on the column.

 

Dynamic binding capacity of Capto MMC at 1 min residence time for three different proteins at different conductivities shown as NaCl concentrationin loading buffer.

 

The Capto™ MMC ligand may interact with proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.

 

TECHNICAL SPECIFICATIONS
Capto MMC media
Ion exchanger type	Multimodal weak cation exchanger
Ionic capacity	0.07-0.09 mmol H+/ml
Average particle size	75 μm
Matrix	Highly cross-linked agarose
Dynamic binding capacity*	> 45 mg BSA/ml medium at 30 mS/cm
Pressure/flow spec.	3 bar at 600 cm/h, 1-m diameter column, 20-cm bed height in water
pH stability	2-14 (short term) 3-12 (long term)
Chemical stability	Stable in all common aqueous buffers:  8 M urea, 6 M Gua-HCl, 70% ethanol.  1 M NaOH† and 1 M acetic acid.
Storage	20% ethanol
Storage temperature	4°C to 30°C
HiTrap prepacked columns
Column volume	1 ml and 5 ml
Column dimensions	0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml)
Max. pressure‡	3 bar (0.3 MPa)
* Dynamic binding capacity at 10% breakthrough as measured at a residence time of 2 min, 300 cm/h in a Tricorn 5/100 column with 10-cm bed height in 50 mM Sodium acetate, pH 4.75, 250 mM NaCl. † No significant change in ionic binding capacity and carbon content after one week storage in 1 M NaOH at 40°C ‡ H2O at 25°C