下图是用阴离子离子交换色谱柱 TSK-GEL  SUGAR AXG预柱反应后测定各类糖类纤维二糖、麦芽糖、乳糖、鼠李糖、来苏糖、核糖、甘露糖、海藻糖、阿拉伯糖、半乳糖、木糖、葡萄糖

Sample:	糖的标准品
Compound:	1) Cellobiose, 2) Maltose, 3) Lactose, 4) Rhamnose, 5) Lyxose, 6) Ribose, 7) Mannose, 8) Fucose, 9) Arabinose, 10) Galactose, 11) Xylose, 12) Glucose

等

1) TSKgel Sugar AXG, 8640, 4.6*150(mmI.D.*mm), (um), 1

流动相 A: 0.6 mol/l borate buffer (pH 7.7)B: 0.7 mol/l borate buffer (pH 7.25) C: 0.7 mol/l borate buffer (pH 8.7) Stepwise gradient

Temperature:70C

Detection:1) FS : ex.: 331 nm em.: 383nm

Injection vol:20ul

Sample load:Each of monosaccharide: 25 umol/lumol/l, disaccharide: 50umol/l

Note:Post column reactionReaction reagent: 2.5% 2-Cyanoacetamide solution Reaction Temperature: 100 C

Title:Separation of saccharides on TSKgel Sugar AXG