17-5162-01	HITRAP ANX FF (HIGHSUB)5 X 1ML
17-5163-01	HITRAP ANX FF(HIGHSUB) 5 X 5ML
17-5193-01	HITRAP PHENYL FF (HS), 5 X 5ML
17-5194-01	HITRAP PHENYL FF (LS), 5X 5ML
17-5195-01	HITRAP PHENYL HP, 5 X 5 ML
17-5196-01	HITRAP OCTYL FF, 5 X 5 ML
17-5197-01	HITRAP BUTYL FF, 5 X 5 ML
17-5247-01	HISTRAP HP 5 X 1 ML
17-5247-05	HISTRAP HP 100 X 1 ML
17-5248-01	HISTRAP HP 1 X 5 ML
17-5248-02	HISTRAP HP 5 X 5 ML
17-5248-05	HISTRAP HP 100 X 5 ML
17-5255-01	HISTRAP FF 5 X 5 ML
17-5255-02	HISTRAP FF 100 X 5 ML
17-5281-01	GSTRAP HP 5 X 1 ML
17-5281-05	GSTRAP HP 100 X 1 ML
17-5282-01	GSTRAP HP 1 X 5 ML
17-5282-02	GSTRAP HP 5 X 5 ML
17-5282-05	GSTRAP HP, 100 X 5 ML
17-5286-01	HISTRAP FF CRUDE 5X5 ML
17-5286-02	HISTRAP FF CRUDE 100X5 ML
17-5319-01	HISTRAP FF 5 X 1 ML
17-5319-02	HISTRAP FF 100 X 1 ML
17-5441-22	HITRAP CAPTO S, 5X1 ML
17-5441-23	HITRAP CAPTO S, 5X5 ML
17-5468-51	HiTrap Capto SP ImpRes 5x1mL
17-5468-55	HiTrap Capto SP ImpRes 5x5mL
17-5470-51	HiTrap Capto Q ImpRes 5x1mL
17-5470-55	HiTrap Capto Q ImpRes 5x5mL
17-6002-33	HITRAP IEX SELECTION KIT
18-1142-75	REC PROTEIN PURIF HANDBOOK
28-4010-81	HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77	HISTRAP FF CRUDE KIT
28-4017-45	GSTRAP 4B, 5 X 1 ML
28-4017-46	GSTRAP 4B, 100 X 1 ML
28-4017-47	GSTRAP 4B, 1 X 5 ML
28-4017-48	GSTRAP 4B, 5 X 5 ML
28-4017-49	GSTRAP 4B, 100 X 5 ML
28-4058-44	HiTrap Capto Adhere 5x1ml
28-4058-46	5x5 ml Hitrap Capto adhere
28-4082-53	HITRAP MABSELECT, 5 X 1 ML
28-4082-55	HITRAP MABSELECT, 1 X 5 ML
28-4082-56	HITRAP MABSELECT, 5 X 5 ML
28-4082-58	HITRAP MABSELECT XTRA, 5X1ML
28-4082-60	HITRAP MABSELECT XTRA, 1X5ML
28-4082-61	HITRAP MABSELECT XTRA, 5X5ML
28-4110-01	HiTrap Butyl HP, 5x1ml
28-4110-05	HiTrap Butyl HP, 5 x 5ml
28-4110-07	HiTrap HIC Selection Kit, 7x1
28-9075-46	StrepTrap HP 5 x 1mL
28-9075-47	StrepTrap HP 1 x 5mL
28-9075-48	StrepTrap HP 5 x 5mL
28-9165-37	HiTrap Capto DEAE, 5X1ML
28-9165-40	HiTrap Capto DEAE, 5X5ML
28-9187-78	MBPTrap 5 x 1mL
28-9187-79	MBPTrap 1 x 5 mL
28-9187-80	MBPTrap 5 x 5mL
28-9343-88	HiTrap Capto IEX Selection Kit
28-9520-85	HiTrap Con A 4B 5 x 1 ml
28-9520-96	HiTrap Con A 4B 5 x 5 ml

HiTrap™ IEX FF Columns

• For method scouting, group separations, concentrations and sample cleanup.
• Convenient and affordable for fast, easy separations either alone or connected in series.
• Prepacked with Q Sepharose™ Fast Flow (strong anion), SP Sepharose™ Fast Flow (strong cation), CM Sepharose™ Fast Flow (weak cation), DEAE Sepharose™ Fast Flow (weak anion), and ANX Sepharose™ 4 Fast Flow (high sub) (weak anion).
• Designed for use with syringe, peristaltic pump, or chromatography systems such as ÄKTA™design.
• High flow rates, and good scale-up potential.

HiTrap™ IEX FF Columns

Technical Information

Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities.

 

ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

 

The active end of the charged group is the same for DEAE Sepharose™ Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose™ Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.

 

TECHNICAL SPECIFICATIONS
Medium	Q Sepharose™ Fast Flow
	SP Sepharose™ Fast Flow
	CM Sepharose™ Fast Flow
	DEAE Sepharose™ Fast Flow
	ANX Sepharose™ 4 Fast Flow (high sub)
Column volume	1 ml or 5 ml
Max. flow rate*
1-ml column	4 ml/min
5-ml column	20 ml/min
Recommended flow rate
1-ml column	1 ml/min
5-ml column	5 ml/min
Max back pressure	3 bar (43 psi, 0.3 MPa)
Storage	20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP)
Storage temperature	4°C to 30°C
* H2O at 25°C

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