17-5053-01	HITRAP Q FF, 5 X 1 ML
17-5054-01	HITRAP SP FF, 5 X 1 ML
17-5055-01	HITRAP DEAE FF 5 X 1 ML
17-5056-01	HITRAP CM FF, 5 X 1 ML
17-5079-01	HITRAP RPROTEIN A FF, 5X1ML
17-5079-02	HITRAP RPROTEIN A FF, 2 X 1 ML
17-5080-01	HITRAP RPROTEIN A FF, 1X5ML
17-5080-02	HITRAP RPROTEIN A FF, 5 X 5 ML
17-5110-01	HITRAP IGM PURIFICATION 5X1ML
17-5111-01	HITRAP IGY PURIFICATION 1X5 ML
17-5112-01	HITRAP STREPTAVIDIN HP5X1 ML
17-5130-01	GSTRAP FF, 5X1ML
17-5130-02	GSTRAP FF, 2X1ML
17-5130-05	GSTRAP FF, 100 X 1 ML
17-5131-01	GSTRAP FF, 1X5ML
17-5131-02	GSTRAP FF 5 X 5 ML
17-5131-05	GSTRAP FF, 100 X 5 ML
17-5143-01	HITRAP BENZAMIDINE FF 5 X 1ML
17-5143-02	HITRAP BENZAMIDINE FF 2 X 1 ML
17-5144-01	HITRAP BENZAMIDINE FF 1 X 5 ML
17-5154-01	HITRAP DEAE FF 5 X 5 ML
17-5155-01	HITRAP CM FF, 5 X 5 ML
17-5156-01	HITRAP Q FF, 5 X 5 ML
17-5157-01	HITRAP SP FF, 5 X 5 ML
17-5158-01	HITRAP Q XL, 5 X 1 ML
17-5159-01	HITRAP Q XL, 5 X 5 ML
17-5160-01	HITRAP SP XL, 5 X 1 ML
17-5161-01	HITRAP SP XL, 5 X 5 ML
17-5162-01	HITRAP ANX FF (HIGHSUB)5 X 1ML
17-5163-01	HITRAP ANX FF(HIGHSUB) 5 X 5ML
17-5193-01	HITRAP PHENYL FF (HS), 5 X 5ML
17-5194-01	HITRAP PHENYL FF (LS), 5X 5ML
17-5195-01	HITRAP PHENYL HP, 5 X 5 ML
17-5196-01	HITRAP OCTYL FF, 5 X 5 ML
17-5197-01	HITRAP BUTYL FF, 5 X 5 ML
17-5247-01	HISTRAP HP 5 X 1 ML
17-5247-05	HISTRAP HP 100 X 1 ML
17-5248-01	HISTRAP HP 1 X 5 ML
17-5248-02	HISTRAP HP 5 X 5 ML
17-5248-05	HISTRAP HP 100 X 5 ML

HiTrap™ IEX FF Columns

• For method scouting, group separations, concentrations and sample cleanup.
• Convenient and affordable for fast, easy separations either alone or connected in series.
• Prepacked with Q Sepharose™ Fast Flow (strong anion), SP Sepharose™ Fast Flow (strong cation), CM Sepharose™ Fast Flow (weak cation), DEAE Sepharose™ Fast Flow (weak anion), and ANX Sepharose™ 4 Fast Flow (high sub) (weak anion).
• Designed for use with syringe, peristaltic pump, or chromatography systems such as ÄKTA™design.
• High flow rates, and good scale-up potential.

HiTrap™ IEX FF Columns

Technical Information

Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities.

 

ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

 

The active end of the charged group is the same for DEAE Sepharose™ Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose™ Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.

 

TECHNICAL SPECIFICATIONS
Medium	Q Sepharose™ Fast Flow
	SP Sepharose™ Fast Flow
	CM Sepharose™ Fast Flow
	DEAE Sepharose™ Fast Flow
	ANX Sepharose™ 4 Fast Flow (high sub)
Column volume	1 ml or 5 ml
Max. flow rate*
1-ml column	4 ml/min
5-ml column	20 ml/min
Recommended flow rate
1-ml column	1 ml/min
5-ml column	5 ml/min
Max back pressure	3 bar (43 psi, 0.3 MPa)
Storage	20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP)
Storage temperature	4°C to 30°C
* H2O at 25°C

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