28-9505-10	HiScreen Q FF
28-9505-11	HiScreen Q HP
28-9505-13	HiScreen SP FF
28-9505-15	HiScreen SP HP
28-9505-16	HiScreen Phenyl HP
28-9505-17	HiScreen IMAC FF
28-9782-43	HiScreen Blue FF
28-9782-44	HiScreen Ni FF
28-9924-72	HiScreen Capto Phenyl (high su
28-9924-74	HiScreen Capto Blue

Technical Information

HiScreen IEX FF columns are prepacked for convenient and robust process development.

 

HiScreen Q FF, HiScreen DEAE FF, and HiScreen SP FF columns are part of the process development platform available from GE Healthcare. The three columns are prepacked with Q Sepharose Fast Flow (strong anion), DEAE Sepharose Fast Flow (weak anion), and SP Sepharose Fast Flow (strong cation) media, respectively. These columns, due to their small column volume and 10 cm bed height, are excellent tools for method optimization, parameter screening, robustness testing, and scaling up. Process fluid velocities can be applied since the 10 cm bed height gives enough residence time, and the results can then serve as a basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Chromatography media prepacked in HiScreen IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.
 

ECHNICAL SPECIFICATIONS
Media	Q Sepharose Fast Flow DEAE Sepharose Fast Flow SP Sepharose Fast Flow
Column volume	4.7 ml
Column dimensions	0.77 × 10 cm
Recommended flow rates	2.3 ml/min (300 cm/h)
Maximum flow rates	4.5 ml/min (450 cm/h)
Maximum pressure over the packed bed during operation, Dp†	1.5 bar (0.15 MPa, 22 psi)
Column hardware pressure limit	8 bar (0.8 MPa, 117 psi)
†Water at room temperature

TECHNICAL SPECIFICATIONS
Media	Q Sepharose Fast Flow DEAE Sepharose Fast Flow SP Sepharose Fast Flow
Column volume	4.7 ml
Column dimensions	0.77 × 10 cm
Recommended flow rates	2.3 ml/min (300 cm/h)
Maximum flow rates	4.5 ml/min (450 cm/h)
Maximum pressure over the packed bed during operation, Dp†	1.5 bar (0.15 MPa, 22 psi)
Column hardware pressure limit	8 bar (0.8 MPa, 117 psi)
†Water at room temperature

 

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