英文名称： HITRAP CHELATING HP
| 17-0408-01 | HITRAP CHELATING HP, 5 X 1 ML |
| 17-0409-01 | HITRAP CHELATING HP, 1 X 5 ML |
| 17-0409-03 | HITRAP CHELATING HP 5 X 5 ML |
| 17-0409-05 | HITRAP CHELATING HP 100 X 5 ML |
| 17-0412-01 | HITRAP BLUE HP, 5 X 1 ML |
| 17-0413-01 | HITRAP BLUE HP, 1 X 5 ML |
| 17-0716-01 | HITRAP NHS-ACTIV HP, 5 X 1ML |
| 17-0716-30 | HiTrap NHS-activated HP, 180 x |
| 17-0717-01 | HITRAP NHS-ACTIV HP, 1 X 5 ML |
| 17-0920-03 | HITRAP IMAC HP, 5 X 1 ML |
| 17-0920-05 | HITRAP IMAC HP, 5 X 5 ML |
| 17-0921-02 | HITRAP IMAC FF, 5 X 1ML |
| 17-0921-04 | HITRAP IMAC FF, 5 X 5 ML |
| 17-0978-13 | HITRAP BUTYL-S FF, 5 X 1ML |
| 17-0978-14 | HITRAP BUTYL-S FF, 5 X 5ML |
| 17-1128-01 | MABTRAP KIT |
| 17-1151-01 | HITRAP SP HP, 5 X 1 ML |
| 17-1152-01 | HITRAP SP HP, 5X5 ML |
| 17-1153-01 | HITRAP Q HP, 5 X 1 ML |
| 17-1154-01 | HITRAP Q HP 5X5 ML |
HiTrap™ Chelating HP Columns
- For purification of histidine-tagged* proteins using different kinds of metal ions†.
- For optimizing purification of (histidine)6-tagged protein purification when Ni2+ is not the best choice of metal ion.
- Prepacked with Chelating Sepharose™ High Performance.
- Purifying proteins with exposed histidine groups via metal ion complex formation.
- Iminidoacetic acid coupled to Sepharose High Performance.
HiTrap™ Chelating HP Columns
Technical Information
)
| TECHNICAL SPECIFICATIONS | |
| Chelating group | Iminodiacetic acid |
| Chelating group coupling linkage | Ether |
| Matrix | Highly cross-linked agarose, 6% |
| Average particle size | 34 μm |
| Metal ion capacity | » 23 μmol Cu2+/ml medium |
| Binding capacity | » 12 mg (histidine)6-tagged protein (Mr 27 600)/ml medium |
| Spacer arm | 7-carbon |
| pH stability | 3-13 (long term), 2-14 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
† For more information see Application Note 18-1145-18: Purification of (histidine)6-tagged proteins using HiTrap™ Chelating HP columns charged with different metal ions.