17-0402-01	HITRAP PROTEIN A HP, 5 X 1 ML
17-0402-03	HITRAP PROTEIN A HP,2 X 1 ML
17-0403-01	HITRAP PROTEIN A HP,1 X 5 ML
17-0403-03	HITRAP PROTEIN A HP, 5 X 5 ML
17-0404-01	HITRAP PROTEIN G HP, 5 X 1 ML
17-0404-03	HITRAP PROTEIN G HP, 2 X 1 ML
17-0405-01	HITRAP PROTEIN G HP,1 X 5 ML
17-0405-03	HITRAP PROTEIN G HP, 5 X 5 ML
17-0406-01	HITRAP HEPARIN HP, 5 X 1 ML
17-0407-01	HITRAP HEPARIN HP,1 X 5 ML
17-0407-03	HiTrap Heparin HP 5x5ml
17-0408-01	HITRAP CHELATING HP, 5 X 1 ML
17-0409-01	HITRAP CHELATING HP, 1 X 5 ML
17-0409-03	HITRAP CHELATING HP 5 X 5 ML
17-0409-05	HITRAP CHELATING HP 100 X 5 ML
17-0412-01	HITRAP BLUE HP, 5 X 1 ML
17-0413-01	HITRAP BLUE HP, 1 X 5 ML
17-0716-01	HITRAP NHS-ACTIV HP, 5 X 1ML
17-0716-30	HiTrap NHS-activated HP, 180 x
17-0717-01	HITRAP NHS-ACTIV HP, 1 X 5 ML
17-0920-03	HITRAP IMAC HP, 5 X 1 ML
17-0920-05	HITRAP IMAC HP, 5 X 5 ML
17-0921-02	HITRAP IMAC FF, 5 X 1ML
17-0921-04	HITRAP IMAC FF, 5 X 5 ML
17-0978-13	HITRAP BUTYL-S FF, 5 X 1ML
17-0978-14	HITRAP BUTYL-S FF, 5 X 5ML

HiTrap™ Protein A HP Columns

• Developed for purification and isolation of monoclonal and polyclonal IgG from ascites, serum, and cell culture supernatants.
• Prepacked with Protein A Sepharose™ High Performance for purification and fractionation of IgG subclasses. The coupling method gives high capacity, high performance, and low leakage levels.
• Fast binding kinetics.
• Binds 20 mg human IgG/ml medium.
• Stable over pH 3-9 for use, and 2-10 for cleaning.

HiTrap™ Protein A HP Columns

Technical Information

TECHNICAL SPECIFICATIONS
Ligand	Native protein A
Ligand coupling method	N-hydroxysuccinimide activation
Ligand density	» 3 mg protein A/ml medium
Matrix	Highly cross-linked agarose, 6%
Average particle size	34 μm
Binding capacity	» 20 mg human IgG/ml medium
Recommended flow rate	1 ml/min (1-ml column) 5 ml/min (5-ml column)
Maximum flow rate	4 ml/min (1-ml column) 20 ml/min (5-ml column)
pH stability	3-9 (long term), 2*-10 (short term)
Storage	20% ethanol
Storage temperature	4°C to 8°C
* pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.

 

Purification of monoclonal mouse IgG_2b on HiTrap™ Protein A HP