FSH beta(1038)
英文名称: FSH beta(1038)
型号:null    抗体货号: sc52332
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: santa

SANTA CRUZ BIOTECHNOLOGY, INC.
FSHβ (1038): sc-52332
Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 http://www.scbt.com
APPLICATIONS
FSHβ (1038) is recommended for detection of FSH∫ of human origin by
immunofluorescence and immunohistochemistry (including paraffinembedded
sections) (starting dilution to be determined by researcher,
dilution range 1:10-1:200).
Suitable for use as control antibody for FSHβ siRNA (h): sc-39315.
Molecular Weight of nonglycosylated FSHβ: 21 kDa.
Molecular Weight of glycosylated FSHβ: 24 kDa.
RECOMMENDED SECONDARY REAGENTS
To ensure optimal results, the following support (secondary) reagents are
recommended: 1) Immunofluorescence: use goat anti-mouse IgG-FITC:
sc-2010 (dilution range: 1:100-1:400) or goat anti-mouse IgG-TR: sc-2781
(dilution range: 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941.
2) Immunohistochemistry: use ImmunoCruz™: sc-2050 or ABC: sc-2017
mouse IgG Staining Systems.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
BACKGROUND
Follicle-stimulating hormone (FSH), also called follitropin, belongs to the family
of glycoprotein hormones that also includes luteininizing hormone and thyroidstimulating
hormone. These hormones are secreted by the pituitary and exist
as heterodimers, consisting of a common α subunit and a homologous but
distinct β subunit. While the α subunit of FSH is involved in the binding of
FSH to the receptor, follicle-stimulating hormone receptor (FSHR), the β subunit
stabilizes this interaction. This heterodimer regulates a variety of processes,
including secretion, post-translational modification and signal transduction.
Both FSH and FSHR are localized to Sertoli cells.
REFERENCES
1. Dias, J.A. 1996. Human follitropin heterodimerization and receptor binding
structural motifs: identification and analysis by a combination of synthetic
peptide and mutagenesis approaches. Mol. Cell Endocrinol. 125: 45-54.
2. Sugahara, T., et al. 1996. Expression of biologically active fusion genes
encoding the common α subunit and either the CG β or FSH β subunits:
role of a linker sequence. Mol. Cell Endocrinol. 125: 71-77.
3. Stanton, P.G., et al. 1996. Structural and functional characterisation of
hFSH and hLH isoforms. Mol. Cell Endocrinol. 125: 133-141.
4. Arnold, C.J., et al. 1998. The human follitropin α subunit C terminus collaborates
with a β subunit cystine noose and an α subunit loop to assemble a
receptor-binding domain competent for signal transduction. Biochemistry 37:
1762-1768.
5. Baccetti, B., et al. 1998. Localization of human follicle-stimulating hormone
in the testis. FASEB J. 12: 1045-1054.
6. Beau, I., et al. 1998. The basolateral localization signal of the folliclestimulating
hormone receptor. J. Biol. Chem. 273: 18610-18616.
CHROMOSOMAL LOCATION
Genetic locus: FSHB (human) mapping to 11p13; Fshb (mouse) mapping to 2 E3.
SOURCE
FSHβ (1038) is a mouse monoclonal antibody raised against isolated human
Follicle Stimulating Hormone.
PRODUCT
Each vial contains 500 μl ascites containing IgG1 with < 0.1% sodium azide.
STORAGE
For immediate and continuous use, store at 4° C for up to one month. For
sporadic use, freeze in working aliquots in order to avoid repeated freeze/
thaw cycles. If turbidity is evident upon prolonged storage, clarify solution
by centrifugation.
PROTOCOLS
See our web site at http://www.scbt.com or our catalog for detailed protocols and
support products.