SuperScript III Reverse Transcriptase,反转录酶 III,18080-044,invitrogen 英文名称: SuperScript III Reverse Transcriptase |
型号:null 产品货号: invitrogen 18080-044 |
价格:请致电:010-57128832,18610462672 |
品牌: invitrogen |
名称:反转录酶 III,SuperScript™ III Reverse Transcriptase 产地:invitrogen 货号: 18080-044 规格:10000u 描述 应用: 第一链 cDNA 合成、阵列标记、cDNA 文库、RT-PCR、引物延伸和 3´ 和 5´ RACE。 来源: 纯化自表达 M-MLV 的 pol 基因的大肠杆菌 (1,2),通过突变增加了热稳定性和半衰期,降低了 RNase H 活性。 性能和质量测试: SDS-PAGE 纯度;脱氧核糖核酸内切酶、脱氧核糖核酸外切酶和核糖核酸酶分析(图 2)及 cDNA 产物的产量和长度。 单位定义: 一个单位的 SuperScript® III RT 是在 37°C 的反应体系中,使用 poly(A)•oligo(dT)12-18 作为模板•引物,在 10 分钟内将 1 纳摩尔脱氧核糖核苷酸掺入酸沉淀材料所需的酶量 (3)。 单位反应条件: 50 mM Tris-HCl (pH 8.3)、40 mM KCl、6 mM MgCl 2、1 mM DTT。 0.5 查看更多mM [ 3 H]dTTP、0.1 mM poly(A)、0.1 mM oligo(dT)12-18、0.1 mg/ml BSA 和酶,共 50 µl,温度 37°C,时间 10 分钟。 Description: SuperScript™ III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript™ II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest View More SuperScript™ III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript™ II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript™ II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript™ III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. The structural modifications provide: Half life of 220 minutes at 50°C for the highest cDNA yields (Figure 1)
Contents and Storage: Ordering Information
|