潮霉素B(Hygromycin B)Roche
型号:null    产品货号: 10843555001
价格:请致电:010-57128832,18610462672
品牌: roche

正规专用发票,签订正规销售合同,全国冷链运输,免费技术支持

潮霉素B(Hygromycin B)Roche
产品名称:潮霉素B
英文名称:hygromycin B 
品牌:Roche  
货号:10843555001
规格:1G
 
定义:潮霉素B由吸水链霉菌(Streptomyces hygroscopicus)产生的一种氨基糖苷类抗生素,通过抑制蛋白质合成能杀死细菌、真菌和高等真核细胞,用于筛选和保持转化有潮霉素抗性基因的原核和真核细胞。 
用途:
1)潮霉素B是氨基糖苷类抗生素,它可以通过抑制蛋白质合成杀死细菌、真菌和高级真核细胞。   
2)潮霉素B通过干扰移位、促进80S核糖体的误译来抑制蛋白质合成。
3)哺乳动物细胞选择的有效浓度通常是50 μg/ml--1 mg/ml之间;对于植物细胞,其有效浓度是20–200 μg/ml;对于细菌,有效浓度是20–200μg/ml;对于真菌,有效浓度是200μg–1 mg/ml。
4)转染中用的特定抗生素。
5)潮霉素B***酸转移酶灭活潮霉素B。
6)携带hph基因转染的细胞对潮霉素有抗性。  
7)转染细胞稳定或暂时表达该基因。
产品描述:
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
For life science research only.
Product Description
Formula: C20H37N3O13
LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
Molecular weight: Mr = 527.5
Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
Contents
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), sterile-filtered
Quality
Purity: >80% (HPLC)